HPLC法测定三种药品中的维生素D2的含量

目的:建立HPLC法测定维D2乳酸钙片、葡萄糖酸钙维D2咀嚼片、维D2磷酸氢钙片三种药品中维生素D2含量的方法。方法:采用Kromasil 100-5-C18(250 mm×4.6 mm,5μm)色谱柱,流动相:乙腈-甲醇(1∶9),检测波长为265 nm,流速:1.0 ml.min-1,柱温30℃。结果:维生素D2在0.005～0.065μg范围内具有良好的线性关系,r=1.000 0。维D2乳酸钙片的回收率为99.4%、RSD=0.4%(n=9),葡萄糖酸钙维D2咀嚼片的回收率为99.6%、RSD=0.5%(n=9),维D2磷酸氢钙片的回收率为99.6%、RSD=0.4%(n=9)。结论:本法快速、简便、准确,可用于上述三种药品中维生素D2的含量测定。

Determination of Vitamin D2 in Three Preparations by HPLC

Objective： To establish a method for the determination of vitamin D2 in calcium lactate tablets,calcium gluconate and vitamin D2 chewable tablets,vitamin D2 and calcium hydrogen phosphate tablets.Method： The HPLC method was performed on a chromatographic column of Kromasil 100-5-C18（250 mm×4.6 mm,5 μm）at 30℃.The mobile phase was composed by 10% of acetonitrile and 90% of methanol.The flow rate was 1.0 ml·min-1,and the detection wavelength was at 265 nm.Result： A good linearity of vitamin D2 was obtained within the range of 0.005-0.065 μg（r=1.000 0） with the recovery of 99.4%（RSD=0.4%,n=9）,99.6%（RSD=0.5%,n=9） and 99.6%（RSD=0.4%,n=9） for vitamin D2 and calcium lactate tablets,calcium gluconate and vitamin D2 chewable tablets and vitamin D2 and calcium hydrogen phosphate tablets,respectively.Conclusion： The method is proved to be rapid,simple and accurate,and applicable for the determination of vitamin D2 in the three preparations.