| Cyclin D1在大鼠肝细胞癌变过程中的异常表达及意义 |
| |
| 引用本文： | 王新红,李宝杰,曲波. Cyclin D1在大鼠肝细胞癌变过程中的异常表达及意义[J]. 哈尔滨医科大学学报, 2003, 37(4) |
| |
| 作者姓名： | 王新红 李宝杰 曲波 |
| |
| 作者单位： | 哈尔滨医科大学第二临床医学院,消化内科,黑龙江,哈尔滨,150086 |
| |
| 摘 要： | 目的 探讨肝细胞癌变过程中cyclinD1 的异常表达及其意义。方法 用 0 .0 5 %的 2 AAF诱导Wistar大鼠建立肝癌动物模型 ,应用免疫组化和原位杂交法检测大鼠肝脏在癌变过程中cyclinD1 的蛋白和mRNA表达。结果 正常组 8只中cyclinD1 蛋白和mRNA表达均正常 ,肝硬化组 16只中有 12只cyclinD1 蛋白和mRNA过表达 ,肝癌组 11例中有 4只cyclinD1 蛋白和mRNA过表达。结论 在 3组中cyclinD1 蛋白和mRNA表达一致 ,无显著差异。肝硬化组和肝癌组的cyclinD1 异常表达均高于正常组 ,而且肝硬化组的cyclinD1 异常表达高于肝癌组。
|
| 关 键 词： | 肝细胞癌 细胞周期蛋白D1 免疫组化 原位杂交 大鼠 |
Expression and significance of cyclin D1 during hepatocellular canceration |
| |
| WANG Xin hong,LI Bao jie,QU Bo. Expression and significance of cyclin D1 during hepatocellular canceration[J]. Journal of Harbin Medical University, 2003, 37(4) |
| |
| Authors: | WANG Xin hong LI Bao jie QU Bo |
| |
| Abstract: | Objective To study the expression and significance of cyclin D 1 during hepatocellular canceration. Methods 0.05% 2 AAF induced rat(Wister) hepatocellular carcinoma. Cyclin D 1 protein and mRNA were examined respectively by immunohistochemistry and in situ hybridization techniques,respectively. Results All of control group showed normal expression of cyclin D 1 protein and mRNA, 12 of 16 liver cirrhosis cases showed overexpression of cyclin D 1 protein and mRNA.Four of 11 hepatocellular carcinoma cases showed overexpression of cyclin D 1 protein and mRNA in the three groups. Conclusion There is no significant difference between expressions of cyclin D 1 protein and mRNA. Cyclin D 1 overexpression in liver cirrhosis and hepatocellular carcinoma shows higher than those in control group. Cyclin D 1 overexpression in liver cirrhosis shows higher than those in hepatocellular carcinoma. |
| |
| Keywords: | hepatocellular carcinoma cyclin D 1 immunohistochemistry in situ hybridization techniques rat |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
