| 多因素复合刺激诱导建立大鼠气虚血瘀证动物模型的探索性研究 |
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| 引用本文： | 任建勋,林成仁,刘建勋,徐立,王敏.多因素复合刺激诱导建立大鼠气虚血瘀证动物模型的探索性研究[J].中国中药杂志,2011,36(1):72-76. |
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| 作者姓名： | 任建勋 林成仁 刘建勋 徐立 王敏 |
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| 作者单位： | 中国中医科学院西苑医院实验研究中心,北京,100091 |
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| 基金项目： | 国家重大新药创制专项(2009ZX09502-017, 2009ZX09502-014, 2009ZX09301-005) |
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| 摘 要： | 目的:应用多因素复合刺激的方法建立大鼠气虚血瘀证动物模型.方法:选用SD大鼠,通过不可预知的慢性睡眠剥夺以及半高脂高糖饮食复合刺激,复制大鼠气虚血瘀证动物模型,检测血液流变学、血脂变化、血管活性分子改变以及血压和心室内压等客观指标方面变化.结果:通过对模型组动物的血液流变学、血脂变化、血管活性分子改变以及血压和心室内压客观指标方面的分析,发现模型大鼠在血瘀证的客观体征上有明显的改变,其中低切变率下的全血黏度明显升高(P<0.05),血浆AngⅡ和ET含量明显升高(P<0.05),血压和室内压有一定下降(P<0.05);与此相对应,经过补阳还五汤干预后前述指标出现明显改变,低切变率下的全血黏度和血浆AngⅡ明显降低(P<0.05),血压和室内压明显升高(P<0.05),表明该模型基本符合目前临床气虚血瘀证候客观变化特点.结论:采用不可预知的慢件睡眠剥夺以及半高脂高糖饮食复合刺激的方法可以成功建立气虚血瘀证大鼠动物模型,该模型的成功有望为将来血瘀证的进一步研究以及活血化瘀方药的筛选提供有益的实验手段.
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| 关 键 词： | 多因素 气虚血瘀 动物模型 补阳还五汤 |
| 收稿时间： | 2010/6/21 0:00:00 |
Experimental study on Qi deficiency and blood stasis induced by muti-factor stimulation in rats |
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| REN Jianxun,LIN Chengren,LIU Jianxun,XU Li and WANG Min.Experimental study on Qi deficiency and blood stasis induced by muti-factor stimulation in rats[J].China Journal of Chinese Materia Medica,2011,36(1):72-76. |
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| Authors: | REN Jianxun LIN Chengren LIU Jianxun XU Li and WANG Min |
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| Institution: | China Academy of Traditional Chinese Medicine, Xiyuan Hospital, Beijing 100091, China;China Academy of Traditional Chinese Medicine, Xiyuan Hospital, Beijing 100091, China;China Academy of Traditional Chinese Medicine, Xiyuan Hospital, Beijing 100091, China;China Academy of Traditional Chinese Medicine, Xiyuan Hospital, Beijing 100091, China;China Academy of Traditional Chinese Medicine, Xiyuan Hospital, Beijing 100091, China |
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| Abstract: | Objective: Animal model of Qi deficiency and blood stasis was established by muti-factor stimulation in rats.Method: Healthy SD rats were chronically stimulated randomly by muti-factor methods(chronic sleep deprivation and feed with moderately high lipid and sugar food).Then the indices of hemorheology,plasma total lipids,vasoactive molecules,blood pressure(BP) and ventricular pressure(VP) were measured.Result: The results as well as the analysis of etiology and pathology suggested that model rats could obj... |
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| Keywords: | muti-factor stimulation Qi deficiency and blood stasis animal model Buyang Huanwu decoction |
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