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| 重组腺病毒AdmIL-12对树突状细胞的基因转染 | |
| 引用本文： | 邵长周,张浩茹,瞿介明,何礼贤,张艳红,王晋慧. 重组腺病毒AdmIL-12对树突状细胞的基因转染[J]. 中国临床医学, 2009, 16(5): 653-656 |
| 作者姓名： | 邵长周 张浩茹 瞿介明 何礼贤 张艳红 王晋慧 |
| 作者单位： | 1. 复旦大学附属中山医院,上海,200032 2. 复旦大学附属华东医院,上海,200040 3. 中国上海生命科学院生物细胞研究所,上海,200032 |
| 基金项目： | 国家自然科学基金(30772019);;上海市重点学科建设项目(B115) |
| 摘 要： | 目的：应用重组腺病毒AdmIL-12转染树突状细胞（DC）制备DC疫苗。方法：以重组腺病毒AdmIL-12转染DC后,用流式细胞仪检测DC的表型,用RT-PCR检测IL-12在DC内的表达,用EILSA法测定细胞培养上清液中的mIL-12和mIFN-γ的含量。通过IL-12刺激T细胞分泌IFN-γ的能力以检测DC分泌的IL-12的生物活性。结果：重组腺病毒AdmIL-12能高效转染DC,转染后DC的表型无显著改变,转染DC能大量分泌具有生物活性的IL-12。结论：腺病毒AdmIL-12成功转染DC,获得有效的DC疫苗。 |
| 关 键 词： | 腺病毒 载体 树突状细胞 细胞因子 疫苗 |
Gene Transfection of Dendritic Cells by Recombinant Adenovirus AdmIL-12 |
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| SHAO Changzhou,ZHANG Haoru,QU Jieming,HE Lixian,ZHANG Yanhong,WANG Jinhui. Gene Transfection of Dendritic Cells by Recombinant Adenovirus AdmIL-12[J]. Chinese Journal Of Clinical Medicine, 2009, 16(5): 653-656 | |
| Authors: | SHAO Changzhou ZHANG Haoru QU Jieming HE Lixian ZHANG Yanhong WANG Jinhui |
| Affiliation: | SHAO Changzhou1 ZHANG Haoru1 QU Jieming2 HE Lixian1 ZHANG Yanhong3 WANG Jinhui3 1. Zhongshan Hospital,Fudan University,Shanghai 200032,China,2. Huadong Hospital,Shanghai 200040,3. Institute of Biochemistry , Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences |
| Abstract: | Objective：To transfect the Dendritic cells （DC） with recombinant adenovirus vector AdmIL-12, make vaccine of DC. Methods：DC was transfected by AdmIL-12 and its phenotype was measured with floweytometery. The expression of IL-12 in DC was valued with PT-PCR method. The value of IL-12 and IFN-γ in culture supernatants was nanlyzed by ELISA. The biological activity of IL-12 secreted from DC was tested by its ability to stimulate T cells to secret IFN-γ. Results： Recombinant adenovirus AdmIL-12 transfected DC effectively. After transfetion, the phenotype of DC had no significant changement,IL-12 was expressed more in DC, DC secreted more IL -12 with biological activity. Conclusion： AdmlL-12 transfeeted DC effectively. The vaccine of DC was made successfully. |
| Keywords: | Adenovirus Vector Dendritic cell Cytokine Vaccine |
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