苦参碱K562细胞早期原癌基因表达的影响

背景与目的：苦参碱具有抗癌活性,我们前期的研究也证明苦参碱对K562细胞有增殖抑制与诱导分化作用。但苦参碱诱导K562细胞多向分化的分子机制尚不清楚。我们拟对其分子机制进行初步研究。方法：采用RT-PCR半定量技术分别检测0.2mg/ml苦参碱对K562细胞原癌基因c-myc、c-jun、H-ras、p21和肝细胞核转录因子HNF-1α表达的影响。结果：经苦参碱处理3h后K562细胞c-myc、c-jun、NHF-1αmRNA表达明显降低,而H-ras、p21mRNA表达明显增高。结论：0.2mg/ml苦参碱引起的K562细胞相关癌基因表达变化可能与K562细胞的增殖抑制和诱导分化有关。

Matrine affects early expression of proto-oncogenes in K562 cells

BACKGROUND & OBJECTIVE: It has been reported that matrine had the anti-tumor activity, and our previous study have provided that matrine had the effects of inhibiting proliferation and inducing differentiation in K562 cell line, but its molecular mechanism is unknown yet. METHOD: The expression of several proto-oncogenes(c-myc, c-jun, H-ras, p21, and HNF-1 alpha) in K562 cell line treated by 0.2 mg/ml matrine were measured by RT-PCR. RESULT: The c-myc, c-jun, and HNF-1 alpha mRNA of K562 cells treated by 0.2 mg/ml matrine were dramatically decreased at the early stage (3 h), while the H-ras and p21 mRNA were increased obviously at the same time. CONCLUSION: The changes of several proto-oncogenes in K562 cells treated by 0.2 mg/ml matrine may be related to inhibiting proliferation and inducing differentiation.