祖师麻总香豆素纯化工艺优选

目的:优化祖师麻总香豆素的纯化工艺.方法:以总香豆素含量为指标,采用静态和动态吸附-洗脱试验筛选大孔树脂型号,通过单因素试验考察最大上样量、洗脱剂浓度和用量、洗脱速度对纯化工艺的影响.结果:AB-8型大孔树脂对总香豆素的吸附性能和解析效果最佳,其最佳纯化工艺为最大上样量3 BV,加去离子水洗脱杂质,用75％乙醇10 BV洗脱,洗脱流速2 BV·h-1.结论:优选的纯化工艺稳定可靠,能满足祖师麻总香豆素渗透泵控释片需求.

Optimization of Purification Technology of Total Coumarins in Daphne giraldii

Objective: To optimize purification technology of total coumarins in Daphne giraldii. Method: With the content of total coumarins as index,model of macroporous resin was screened by static and dynamic adsorption-elution test,effect of the maximum sample volume,the concentration and amount of eluent,elution speed on purification technology was investigated by single-factor test. Result: AB-8 resin showed the best due to its adsorption and desorption properties,optimum purification conditions were as follows:the maximum sample volume 3 BV,added deionized water for removing impurities,and eluted with 10 BV of 75% ethanol at the flow rate of 2 BV·h^-1. Conclusion: This optimized technology was stable and reliable,which could meet requirements of total coumarins in D. giraldii osmotic pump tablets.