|
|||||
|
|
Differential dynamics of donor DC and non-DC peripheral blood mononuclear cell microchimerism in lung transplantation |
|
| Authors: | Joel Van der Meulen Nicole A Mifsud Diahnn Abud Eldho Paul Michael D Varney Sharon R Lewin Paul U Cameron Tom C Kotsimbos |
| Institution: | aDepartment of Medicine, Monash University, Melbourne, Australia;bVictorian Transplantation and Immunogenetics Service, Melbourne, Australia;cDepartment of Allergy, Immunology and Respiratory Medicine, The Alfred Hospital, Melbourne, Australia;dInfectious Diseases Unit, The Alfred Hospital, Melbourne, Australia;eDepartment of Immunology, Monash University, Melbourne, Australia;fMonash Institute of Health Services Research, school of Public Health and Preventative Medicine, Monash University, Australia |
| Abstract: | Donor cell microchimerism induces tolerance in animal models and may increase graft survival in man. Since dendritic cells (DC) are critical for induction of both tolerance and alloreactivity we developed a method to quantitate microchimerism in donor DC and non-DC in peripheral blood mononuclear cells (PBMC) after lung transplantation. Longitudinal analysis of donor cell microchimerism in eleven sex mismatched lung transplant recipients (LTR) up to 12 months post-transplant used Y chromosome based real-time PCR on sorted cells. Total DC or a proportion of DC subsets in PBMC did not change but there were heterogeneous and dynamic changes in microchimerism in DC and non-DC. Analysis of changes in DC using a mixed model analysis showed significantly less reduction in DC compared to non-DC over time (0.49, p = 0.001). Preferential DC persistence compared to non-DC may indicate tolerance induction but future studies are required to determine if DC microchimerism after transplantation affects clinical outcomes. |
| Keywords: | Dendritic cells Lung transplantation Microchimerism Tolerance |
| 本文献已被 ScienceDirect 等数据库收录！ | |