Polar body diagnosis of common aneuploidies by FISH |
| |
Authors: | Yury Verlinsky Jeanine Cieslak Michael Freidine Victor Ivakhnenko George Wolf Larissa Kovalinskaya Melody White Aaron Lifchez Brian Kaplan Jacob Moise Jorge Valle Norman Ginsberg Charles Strom Anver Kuliev |
| |
Institution: | (1) Reproductive Genetics Institute, Illinois Masonic Medical Center, 836 Wellington Avenue, 60657 Chicago, Illinois |
| |
Abstract: | Purpose: The purpose of this work was to investigate the reliability and accuracy of polar body analysis for preimplantation diagnosis of common aneuploidies in IVF patients of advanced maternal age.
Design: We have previously introduced polar body analysis as an approach for nondestractive evaluation of the genotype of human oocytes. The method has recently been applied in a clinical trial involving 45 infertile patients, demonstrating the feasibility of preconception diagnosis of common aneuploidies by fluorescent in situ hybridization (FISH). The present paper describes the experience of polar body diagnosis in 135 IVF patients (161 cycles) of advanced maternal age.
Results: FISH results of the first and/or second polar bodies were available in 648 (72.4%) of 895 biopsied oocytes subjected to FISH analysis. Of 648 oocytes with FISH results, 208 demonstrated chromosomal abnormalities. Of 440 oocytes predicted to be free from monosomy or trisomy of chromosomes X, 18, and/or 13/21, 314 were normally fertilized, cleaved, and transferred in 122 treatment cycles, resulting in 6 healthy deliveries and 12 ongoing pregnancies following confirmation of the polar body diagnosis by CVS or amniocentesis.
Conclusion: The method may be useful for detection of oocytes with common chromosomal trisomies in IVF patients of advanced maternal age.Presented at the 5th Annual Meeting of the International Working Group on Preimplantation Genetics, Hamburg, Germany, June 28, 1995. |
| |
Keywords: | human first polar body human second polar body fluorescent in situ hybridization (FISH) preimplantation diagnosis chromosomal aneuploidies |
本文献已被 SpringerLink 等数据库收录! |
|