核因子-κB传导通路与舌癌细胞平阳霉素化疗敏感性的关系

的 探讨核因子-κB（NF-κB）/p65信号传导通路与Tca8113细胞平阳霉素化疗敏感性的关系。方法 以脂质体为载体，转染2 mg/L的p65反义寡核苷酸于Tca8113细胞 后，8 mg/L的平阳霉素处理细胞，3 h、6 h后免疫组化、Western blot法检测细胞核内p65形态和含量变化，48 h后MTT法检测细胞抑制率。结果 平阳霉素能激活细胞内的NF-κB/p65信号传导通路，而p65反义寡核苷酸转染能明显抑制该信号通路，在6 h时胞核内p65含量显著减少（P<0.05），48 h时细胞抑制率明显提高（P<0.05）。结论 抑制NF-κB/p65信号传导通路能够提高Tca8113细胞平阳霉素化疗敏感性。

Relationship between Nuclear Factor Kappa B Signal Transduction Way and the Sensitivity of Tongue Carcinoma Cell to Pingyangmycin Chemotherapy

OBJECTIVE: To examine the relationship between NF-kappaB signal transduction way and the sensitivity of Tca8113 carcinoma cell to Pingyangmycin chemotherapy. METHODS: 2 mg/L antisense p65 oligodeoxynucle-otides (5'-GAACAGTTCGTCCATGGCCG-3') was transfected into Tca8113 cells through Lepofectin vectin, then the cells were treated with 8 mg/L Pingyangmycin. After 3 h and 6 h, the expression of p65 in nucleus was studied with immunohistochemical methods and Western blot analyses. After 48 h, the inhibitory rate of cell growth was detected with MTT assay. RESULTS: The NF-kappaB/p65 signal transduction way in Tca8113 cells was activated after treated with Pingyangmycin. But the transfection of antisense p65 oligodeoxynucle-otides inhibited the activation of the signal transduction, the p65 expression in nucleus was decreased significantly (P < 0.05) at 6 h, and the inhibitory rate of cell growth was increased significantly (P < 0.05) at 48 h. CONCLUSION: The results suggested the chemotherapeutic sensitivity of Tca8113 carcinoma cell to Pingyangmycin was improved by the inhibition of NF-kappaB/p65 signal transduction.