“生精散”对大鼠睾丸扭转复位后生精功能的影响及其机制研究

目的:研究"生精散"对大鼠睾丸扭转复位后生精功能的影响及其机制。方法:40只雄性SD大鼠随机分为假手术组(A组),对照组(B组),生精散组小(C组)、中(D组)、大剂量组(E组),每组8只。建立左侧睾丸扭转模型,B组自扭转前1 h开始给予每日灌胃生理盐水1 ml/d,C组(0.01 g/kg.d)、D组(0.02 g/kg.d)、E组(0.03 g/kg.d)分别按体重给予灌服生精散,连续35 d后处死大鼠,对大鼠进行精液常规分析,用RT-PCR检测大鼠精子CatSper1的表达。结果:a+b级精子百分率、精子存活率、精子浓度,CatSper1基因表达,与A组[(51.30±6.60)%、(69.01±7.20)%、(40.53±7.01)×106/ml、2.04±0.77]相比,B组[(15.30±6.30)%、(44.42±6.36)%、(21.00±6.14)×106/ml、1.12±0.50),均显著降低(P<0.01);与B组相比,D组[(51.63±3.20)%、(72.09±2.20)%、(55.30±5.90)×106/ml、2.11±0.20]、E组[(55.93±3.17)%、(73.01±2.11)%、(58.33±4.90)×106/ml、2.31±0.17]均显著升高(P<0.01),而C组[(18.02±0.23)%、(48.04±7.01)%、(22.87±2.10)106/ml、1.19±0.51]升高不明显(P>0.05)。结论:生精散可以促进睾丸扭转复位后精子质量的恢复,其机制可能与调节生精功能,提高精子细胞CatSper1基因的表达有关。

Improving effect of Shengjingsan on spermatogenic function following testicular torsion/detorsion in rats and its mechanism

Objective: To study the effect of Shengjingsan on spermatogenic function following testicular torsion/detorsion in rats and its action mechanism.Methods: Forty SD male rats were equally randomized to groups A(sham operation),B(control),C(low-dose Shengjingsan),D(medium-dose Shengjingsan) and E(high-dose Shengjingsan).The model of testicular torsion was established by 720° clockwise torsion of the left testis for 4 hours.An hour before operation,the rats of group B received daily gavage of normal saline at 1 ml per kg per d,while those in groups C,D and E that of Shengjingsan at 0.01,0.02 and 0.03 g per kg per d,all for 35 days.Then all the rats were sacrificed for measuring the semen parameters by CASA and detecting the expression of the CatSper1 gene in the sperm by RT-PCR.Results: Compared with group A,Sperm concentration,the percentage of grade a+b sperm,sperm vitality and CatSper1 expression were significantly lower in group B([15.30 ± 6.30]%,[44.42 ± 6.36]%,[21.00 ± 6.14] ×106/ml and 1.12 ± 0.50) than in A([51.30 ± 6.60]%,[69.01 ± 7.20]%,[40.53 ± 7.01] ×106/ml and 2.04 ± 0.77)(P < 0.01).Compared with group B,the four parameters were increased remarkably in groups D([51.63 ± 3.20]%,[72.09 ± 2.20]%,[55.30 ± 5.90] ×106/ml and 2.11 ± 0.20) and E([55.93 ± 3.17]%,[73.01 ± 2.11]%,[58.33 ± 4.90] ×106/ml and 2.31 ± 0.17)(P < 0.01),but not significantly in C([18.02 ± 0.23]%,[48.04 ± 7.01]%,[22.87 ± 2.10] ×106/ml and 1.19 ± 0.51)(P > 0.05).Conclusion: Shengjingsan can improve sperm parameters following testicular torsion/detorsion in male rats by regulating their spermatogenic function and improving the expression of CatSper1 in the sperm.