精液液化异常与精浆脂蛋白a的检测和分析

目的:通过对男性不育症患者精液液化异常的精浆进行脂蛋白a检测和分析,探讨精浆脂蛋白a对精液液化异常的影响及临床意义。方法:按照WHO《人类精液检查与处理实验室手册》第5版标准,对101例精液液化异常者、26例精液液化正常对照者的精液进行常规分析,再加糜蛋白酶于精液中置于37℃孵育30 min,待挑起完全无丝状物后离心取上层精浆检测脂蛋白a。结果:在101例精液液化异常者中精浆脂蛋白a水平(526.2±243.5)mg/L]明显高于26例精液液化正常对照组精浆脂蛋白a水平(296.9±105.2)mg/L](P<0.01)。结论:机体脂蛋白a具有抑制纤维蛋白溶解功能,精液液化异常中纤维蛋白的溶解延迟可能与精浆脂蛋白a水平增高有一定相关性。临床诊断精液液化异常引起的男性不育症时,应考虑精浆脂蛋白a水平的影响。

Abnormal semen liquefaction and seminal plasma lipoprotein(a)

Objective: To explore the effect of seminal plasma lipoprotein(a) in abnormal semen liquefaction and its clinical significance.Methods: According to The WHO Laboratory Manual for the Examination and Processing of Human Semen,we conducted semen routine analyses of 101 patients with abnormal semen liquefaction and 26 normal healthy controls.We added chymotrypsin to the semen for 30 minutes of incubation at 37 ℃.When there were filaments,we centrifuged the semen and obtained the upper seminal plasma to determine the level of lipoprotein(a).Results: The level of lipoprotein(a) was significantly higher in the 101 patients(526.2 ± 243.5] mg/L) than in the 26 normal controls(296.9±105.2] mg/L)(P<0.01).Conclusion: Lipoprotein(a) can inhibit fibrin dissolution,and delayed fibrin dissolution in semen liquefaction may be related to the increased level of seminal plasma lipoprotein(a).The seminal plasma lipoprotein(a) level should be taken into account in the clinical diagnosis of male infertility caused by abnormal semen liquefaction.