影响人类精子耐冻性的蛋白质分析

目的:利用比较蛋白质组学技术研究影响人类精子耐冻性的蛋白质。方法:将10例供精志愿者的31份精液标本依据冷冻复苏率的不同分为高冷冻复苏率组和低冷冻复苏率组,应用二维电泳技术和质谱分析鉴定技术寻找两组标本的精子蛋白和精浆蛋白在表达量上的差异。结果:在精子蛋白和精浆蛋白中共发现了22个差异表达蛋白。其中精浆蛋白12个,分别为细胞角蛋白1、神经元导向因子3、Rho2样蛋白、Krueppel样因子10、FERM域蛋白8、GMP合成酶、鸟嘌呤结合蛋白G、溶质载体家族12、ATP结合盒亚家族D1、6-磷酸果糖-2-激酶、肌钙蛋白C、肌球蛋白轻链;精子蛋白9个,分别为卷曲螺旋结构域蛋白113、RAF原癌基因丝氨酸/苏氨酸蛋白激酶、Ras相关蛋白Rab-31、α微管蛋白3C/D、锌指蛋白879、胆固醇26-羟化酶、DNA修复和重组蛋白、细胞角蛋白73、钙磷蛋白;精浆和精子共有蛋白1个,为细胞周期依赖性蛋白激酶1。文献调研发现这些差异蛋白主要与精子的能量代谢、成熟、运动、DNA修复等有关。结论:鉴定了多种可能影响人类精子耐冻性的蛋白质,为以后从分子水平上揭示精子冷冻复苏原理提供了依据。

Proteins in sperm and seminal plasma associated with human sperm resistance to cryopreservation

Objective: To identify the proteins that could improve the resistance of human sperm to cryopreservation using comparative proteomics.Methods: A total of 31 semen samples from 10 donors were divided into a high recovery and a low recovery group.Differentially expressed proteins in sperm and seminal plasma were detected and compared between the two groups by two-dimensional differential gel electrophoresis and mass spectrometry.Results: Totally,22 differentially expressed proteins were found in the two groups,12 seminal plasma proteins,9 sperm proteins,and 1 belonging to both.These identified proteins were involved in the maturation,movement,energy metabolism,DNA repair and other activities of spermatozoa.Conclusion: Many proteins were identified in sperm and seminal plasma that might influence the resistance of human sperm to cryopreservation.