Characteristics of a micro-biolistic system for murine immunological studies |
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| Authors: | Y Liu N K Truong M A F Kendall B J Bellhouse |
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| Institution: | (1) Oxford Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford, OX2 6PE, UK;(2) Graduate School of Biomedical Engineering, University of New South Wales, Sydney, New South Wales, 2052, Australia;(3) Australian Institute for Bioengineering and Nanotechnology (AIBN), Queensland Bioscience Precinct, The University of Queensland, Brisbane, Queensland, 4072, Australia |
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| Abstract: | With an advanced computational fluid dynamics (CFD) technique, we have numerically developed and examined a micro-biolistic
system for delivering particles to murine target sites. The micro-particles are accelerated by a high speed flow initiated
by a traveling shock wave, so that they can attain a sufficient momentum to penetrate in to the cells of interest within murine
skin (or mucosa). In immunization application, powdered vaccines are directly delivered into the antigen presenting cells
(APCs) within the epidermis/dermis of the murine skin with a narrow and highly controllable velocity range (e.g., 699±5.6 m/s
for 1.8 μm modeled gold particles) and a uniform spatial distribution over a diameter of ~ 4 mm target area. Key features of gas dynamics
and gas-particle interaction are presented. Importantly, the particle impact velocity conditions are quantified as a function
of: stand-off distance (2–15 mm), driver gas species (air/helium mixtures), particle density (1,050 kg/m3 and 19,320 kg/m3) and particle size (1–5 μm for gold particles and 10–50 μm for less dense particles, respectively). The influential parameters—representative of immunotherapeutic (e.g., DNA vaccination)
and protein (e.g., lidocaine) biolistic applications—are studied in detail. |
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| Keywords: | Biolistic DNA vaccination Gene gun Impact Micro-particle Murine Simulation |
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