结核患者外周血PBMC中T-bet和Eomes表达的检测

目的初步探讨T-bet和Eomes的表达与结核病发生的关系。方法分析比较结核患者(TB)与健康对照(HC)、结核菌潜伏感染者(LTBI)以及细菌性肺炎患者(PN)外周血PBMC中T-bet和Eomes的m RNA表达水平,并观察结核患者在抗结核治疗前及抗结核治疗3、6、9和12个月后,外周血PBMC中T-bet和Eomes的m RNA表达水平的变化。结果结核患者(TB)外周血PBMC中T-bet和Eomes的m RNA表达水平显著低于健康对照(HC)、结核菌潜伏感染者(LTBI)以及细菌性肺炎患者(PN)(P0.05)。同时,在抗结核治疗12个月后,T-bet的表达水平较治疗前显著升高(P0.05),Eomes的表达水平较治疗前升高,但差异无统计学意义(P0.05)。结论 T-bet和Eomes两个基因表达水平的下降可能与结核病的发生有关。

Detection of T-bet and Eomes in PBMC of patients with tuberculosis

Objective To study the relationship between the T-bet/ Eomes and the occurrence of tuberculosis.Methods Comparative analysis of expression of PBMC T-bet/Eomes mRNA in tuberculosis (TB),healthy controls (HC),latent tuberculosis infection (LTBI) and bacterial pneumonia (PN).Then,we observed the changes of T-bet/Eomes expression before (0 m) and after 3 months,6 months,9 months and 12 months anti-tuberculosis therapy in the patients with tuberculosis cases.Results Expression of PBMC T-bet and Eomes in tuberculosis (TB) were significantly lower than those of healthy controls (HC),latent tuberculosis infection (LTBI) and bacterial pneumonia (PN) (P＜0.05).At the same time,the expression level of T-bet was significantly increased after anti-tuberculosis treatment for 12 months (P＜0.05),Eomes also increased but there was no significant difference (P＞0.05).Conclusion The decreased expression of T-bet and Eomes may be related to the occurrence of tuberculosis..