大蒜素对小鼠外周血淋巴细胞的影响

目的探索大蒜素对小鼠外周血淋巴细胞的影响。方法将40只清洁级昆明小鼠随机分为生理盐水对照组[0.1 mL生理盐水/(10 g·bw)]、大蒜素低剂量组[100 mg/(kg·bw)]、大蒜素中剂量组[200 mg/(kg·bw)]和大蒜素高剂量组[400 mg/(kg·bw)],每组10只,每天灌胃1次,灌胃容量0.1 mL/10 g,连续灌胃14 d后,随机选取8只,采用摘眼球的方式进行取血,制取为单细胞悬液,用流式细胞仪进行检测,分析大蒜素对于小鼠外周血中的CD4~+T细胞、CD8~+T细胞及CD4~+/CD8~+值及淋巴细胞周期的影响。结果高剂量组及中剂量组与对照组相比淋巴细胞亚群和周期均出现了显著性差异,CD4~+T细胞、CD4~+/CD8~+值相对提高,CD8~+T细胞值降低(P0.05);G_2/M期及S期的细胞的比例增加,G_0/G_1期的细胞的比例降低(P0.05)。结论大蒜素具有一定的免疫调节功能,尤其中、高浓度的大蒜素通过对外周血淋巴细胞周期的阻滞作用,使外周血淋巴细胞处于较高的增殖状态,促进淋巴细胞亚群的分化,激化小鼠的免疫功能,对小鼠的细胞免疫和体液免疫功能具有增强作用。

Effects of allicin on peripheral blood lymphocytes in mice

Objective To explore the effects of allicin on peripheral blood lymphocytes in mice.Methods Forty Kunming mice of clean grade were randomly divided into 4 groups,including a saline group [0.1 mL NC/(10 g·bw)],an allicin low dose group [100 mg/(kg·bw)],an allicin middle dose group [200 mg/(kg·bw)] and an allicin high dose group [400 mg/(kg·bw)],with 10 rats in each group,and the gastric perfusion with volume 0.1 mL/10 g was given,once a day for 14 days.Eight rats were randomly selected to take blood by the removing eye approach to prepare the single cell suspension,and it was detected by flow cytometry to explore the effects of allicin on peripheral blood CD4+T cells,CD8+T cells and CD4+/CD8+ value,and peripheral blood lymphocyte cell cycle.Results Compared with the control group,the lymphocyte subsets and cycles in the high dose group and medium dose group had significant differences with the relative increases of CD4+T cells and CD4+/CD8+,but the decrease of CD8+T cell values (all P＜0.05).The proportion of cells in G2/M phase and S phase increased,but the proportion of cells in G0/G1 phase decreased (P＜0.05).Conclusions Allicin has a certain immune regulation function,especially the middle and high concentration of allicin can enhance the cellular immunity and humoral immune function in mice,through the role of peripheral blood lymphocyte cell cycle arrest in the peripheral blood lymphocytes with the higher proliferation to promote the differentiation of lymphocyte subsets and intensify the immune function.