深圳市龙岗区空肠弯曲菌的流行状况及分子分型

目的了解并分析深圳市龙岗区腹泻患者粪便、禽肉类等样品中空肠弯曲菌的流行状况及同源性。方法对2016年5—12月深圳市龙岗区某医院腹泻患者粪便或肛拭子样品和来自养鸡场、农贸市场、超市等地的禽畜类等样品进行空肠弯曲菌分离培养,菌株经镜检和生化鉴定后再使用脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)对其进行全基因组分子分型。最后应用Bio Numerics软件进行聚类分析。结果 41份腹泻患者样品分离出5株空肠弯曲菌,分离率为12.2%,全部阳性样品均来自11岁以下儿童。53份来自食品和外环境样品分离出14株空肠弯曲菌,分离率为26.4%,其中来自农贸市场的分离率为57.1%(8/14),来自不同超市样品空肠弯曲菌的分离率为11.1%(4/36)。18株菌呈现15种PFGE带型,其中来自市场1的3株菌株带型完全一致,且与病例1只有一条带的差异;来自市场2与病例5的带型完全一致且与病例4的带型只有一条带的差异。结论深圳市龙岗区腹泻患者空肠弯曲菌感染率较高,感染可能是由于食用了来自市场未煮熟的食物或接触了来自市场被空肠弯曲菌污染的食物,农贸市场存在空肠弯曲菌的交叉污染,该地区空肠弯曲菌呈现多样性。

Prevalence and molecular typing of Campylobacter jejuni in Longgang District,Shenzhen

Objective To understand the prevalence and homology analysis of Campylobacter jejuni in samples of diarrhea patients and poultry meat in Longgang District,Shenzhen.Methods C.jejuni was isolated and cultured from stool and anal swab samples of diarrhea patients from a hospital in Longgang District of Shenzhen during May to December,2016,and samples of poultry and livestock from chickens,farmers' markets and supermarkets.The whole genome of the isolates was analyzed by pulsed field gel electrophoresis after the isolates were examined by microscopy and biochemistry.Finally,BioNumerics software was used for cluster analysis.Results Five C.jejuni isolates were isolated from 41 diarrhea patients samples,the isolation rate was 12.2％.All positive samples were from children under 11 years of age.14 C.jejuni isolates were isolated from 53 food and outer environment samples,the isolation rate was 26.4％.The separation rate from the farmer's market was 57.1％ (8/14).That of C.jejuni from different supermarket samples was 11.1％ (4/36).18 isolates showed 15 PFGE patterns.PFGE pattern of three isolates from the market 1 was identical and had one band difference from that of case 1.The isolates from the market 2 and case 5 were exactly the same PFGE pattern and there was only one band difference between this pattern and that of case 4.Conclusion The C.jejuni infection rate was high in diarrhea patients in Longgang District,Shenzhen.Infection may be due to consumption of uncooked food from markets or contact with markets from the contaminated food with C.jejuni.Farmer markets exist C.jejuni cross-contamination.A variety of C.jejuni was present in this area.