Dishevelled-1、Cyclin D1和CD44v6在儿童骨肉瘤中的表达及临床意义

目的 探讨Dishevelled-1（Dvl1）、Cyclin D1和CD44v6蛋白在儿童骨肉瘤组织中的表达及其与骨肉瘤临床病理特征的关系。方法 采用免疫组化法检测Dvl1、Cyclin D1和CD44v6在47例儿童骨肉瘤组织中的表达情况。分析3者表达的关系及其与临床病理特征的关系。结果 Dvl1 、Cyclin D1和CD44v6在儿童骨肉瘤组织中的高表达率为76.60％（36／47）、72.34％（34／47）和68.09％（32／47）。Dvl1表达与肿瘤直径（P=0.020）和Ennecking分期（P=0.032）有关； Cyclin D1和CD44v6的表达也分别与肿瘤直径（P=0.007，P=0.036）和Ennecking分期（P=0.024，P=0.020）有关。Dvl1表达与Cyclin D1（r=0.332，P=0.023）和CD44v6表达（r=0.376，P=0.009）呈正相关。结论 Dvl1、Cyclin D1和CD44v6在儿童骨肉瘤中呈高表达，其高表达能够促进儿童骨肉瘤的恶性进展。

The expression of Dishevelled-1,Cyclin D1 and CD44v6 in pediatric osteosarcoma tissues and their relation-ships with clinical pathological parameters

Objective To examine the expression of Dishevelled-1 （Dvl1）， Cyclin D1 and CD44v6 in pediatric osteosarcoma tissues， and investigate the correlations among them and the clinicopathological factors. Methods The expressions of Dvl1， Cyclin D1 and CD44v6 were detected in 47 pediatric osteosarcoma tissues by immonohistochemistry method. The relationship among Dvl1， Cyclin D1， CD44v6 and clinicopathological factors was investigated. Results The high expression rates of Dvl1，Cyclin D1 and CD44v6 were 76.60％ （36／47）， 72.34％ （34／47） and 68.09％ （32／47）， respectively in pediatric osteosarcomas. The expression of Dvl1 was correlated with the tumor diameter （P=0.020） and Ennecking stage （P=0.032）. Similarly， the expression of Cyclin D1 and Cd44v6 were also correlated with the tumor diameter （P=0.007 and P=0.036） and Ennecking stage （P=0.024 and P=0.020） of pediatric osteosarcomas. Moreover， the expression of Dvl1 was positively correlated with Cyclin D1 expression （r=0.332，P=0.023） and CD44v6 expression （r=0.376，P=0.009）， respectively. Conclusion Dvl1， Cyclin D1 and CD44v6 were highly expressed in pediatric osteosarcoma. The high expressions of Dvl1， Cyclin D1 and CD44v6 can promote the malignant progression of pediatric osteosarcomas.