非小细胞肺癌组织中lncRNA HOTAIR的表达及临床意义

目的 探讨长链非编码lncRNA HOTAIR在非小细胞肺癌（NSCLC）组织中的表达及其临床意义。方法 收集本院2010年1月至2013年1月经病理组织学确诊的91例NSCLC患者，取其经手术切除癌组织91例和配对癌旁组织标本62例，采用实时荧光定量PCR（QPCR）法检测以上标本的HOTAIR水平，分析NSCLC HOTAIR水平与临床病理特征（性别、年龄、TNM分期、肿瘤大小、分化程度、淋巴结转移、病理类型及CEA水平）的关系，并分析不同HOTAIR水平的预后情况。结果 NSCLC组织的HOTAIR相对表达量为6.271±0.884，高于癌旁组织的1.027±0.134，差异有统计学意义（P＜0.05）；NSCLC组织中HOTAIR表达与性别、年龄、分化程度、病理类型及CEA异常均无关，而与TNM分期、肿瘤大小及淋巴结转移有关，其中Ⅱ+Ⅲ期、肿瘤大小＞3 cm及有淋巴结转移者的HOTAIR表达均高于对应项，差异均有统计学意义（P＜0.05）。91例NSCLC患者的中位总生存期（OS）为36.4个月，与性别、年龄、分化程度、病理类型及CEA水平无关，但与TNM分期、肿瘤大小、淋巴结转移及HOTAIR表达有关，其中HOTAIR高表达者中位OS为27.8个月，低于低表达者的36.4个月，差异有统计学意义（P＜0.05）。多因素分析显示，TNM分期、肿瘤大小、淋巴结转移及HOTAIR表达是影响NSCLC预后的独立因素。结论 HOTAIR在NSCLC组织中高表达，且与TNM分期、肿瘤大小、淋巴结转移及预后均有关，可能在NSCLC的发生发展中有一定作用。

Expression of lncRNA HOTAIR in non-small cell lung cancer and its clinical significance

Objective To investigate the expression level and clinical significance of long chain non encoded lncRNA HOTA IR in non-small cell lung cancer （NSCLC）. Methods Ninety-one cases of NSCLC patients diagnosed by pathology from January 2010 to January 2013 in our hospital were enrolled. Ninety-one cases of surgical resected cancer tissues and 62 cases of paired adjacent tissue samples were collected. HOTAIR levels were detected by quantitative real-time PCR （QPCR） method. The relationship between HOTAIR level in NSCLC tissues and clinical pathological parameters （gender， age， TNM stage， tumor size， differentiation， lymph node metastasis， pathologic type and CEA level） were analyzed. The prognosis of different HOTAIR levels was analyzed according to the follow-up data. Results The relative level of HOTAIR in NSCLC tissues was 6.271±0.884， higher than that of adjacent cancer tissue with statistical significance （P＜0.05）. The expression of HOTAIR in NSCLC was not related to gender， age， degree of differentiation， pathological type and CEA abnormality， but related with TNM stage， tumor size and lymph node metastasis. The levels of HOTAIR in patients of TNM stage Ⅱ-Ⅲ， tumor size more than 3 cm or with lymph node metastasis were higher than those of corresponding items （P＜0.05）. The median overall survival（OS） of 91 patients was 36.4 months， unrelated with gender， age， degree of differentiation and serum CEA level， but related with TNM stage， tumor size， lymph node metastasis and HOTAIR expression. The median OS of patients with HOTAIR high-expression was 27.8 months， lower than 36.4 months of patients with low-expression with statistical significance （P＜0.05）. Multivariate analysis showed that TNM stage， tumor size， lymph node metastasis and HOTAIR expression were independent factors influencing the prognosis of NSCLC patients. Conclusion HOTAIR is highly expressed in NSCLC tissues， and it may be related to TNM stage， tumor size， lymph node metastasis and prognosis. It may paly a role in the occurrence and development of NSCLC.