SMG 1、mTOR和Raptor在乳腺癌组织中的表达及临床意义

目的 探讨磷脂酰肌醇-3-激酶相关激酶（PIKK）家族成员生殖器形成抑制基因1（SMG 1）、哺乳动物雷帕霉素靶蛋白（mTOR）及mTOR调节相关蛋白（Raptor）在乳腺癌组织中的表达及临床意义。方法 收集2014年1月至2015年7月乳腺癌组织68例及配对癌旁组织40例和乳腺纤维腺瘤组织40例，采用免疫组化SP法检测以上组织中SMG 1的表达情况，实时定量PCR（QPCR）检测mTOR和Raptor的表达水平，分析3者表达与乳腺癌临床病理特征的关系，并采用Spearman法分析3者表达的相关性。结果 乳腺癌组织中SMG 1的阳性表达率为27.9％（19／68），低于癌旁组织的77.5％（31／40）和乳腺纤维腺瘤组织的82.5％（33／40），差异有统计学意义（P＜0.05）；乳腺癌组织中mTOR和Raptor的相对表达量分别为2.754±0.213和4.137±0.626，均高于癌旁组织和乳腺纤维腺瘤组织（P＜0.05）。SMG 1表达与乳腺癌的肿瘤大小、淋巴结转移及组织学分级均有关（P＜0.05），mTOR表达与乳腺癌的肿瘤大小、TNM分期及组织学分级均有关（P＜0.05），Raptor表达与乳腺癌的肿瘤大小、淋巴结转移及组织学分级均有关（P＜0.05）；SMG 1表达与mTOR和Raptor表达呈负相关（r=-0.547、r=-0.415， P＜0.05），mTOR和Raptor表达呈正相关（r=0.664， P＜0.05）。结论 乳腺癌组织中mTOR、Raptor呈高表达而SMG 1呈低表达，3者表达均与乳腺癌的肿瘤大小、组织学分级有关，提示PIKK家族成员SMG 1、mTOR和Raptor可能在乳腺癌发生、发展中具有重要意义。

Expression and clinical significance of SMG 1, mTOR and Raptor in breast cancer tissues

Objective To investigate the expression of suppressor with morphogenetic effect on genitalia-1（SMG 1），mammalian target of rapamycin（mTOR）and regulatory associated protein of mTOR（Raptor）in breast cancer and analyze their clinical significance. Methods From January 2014 to July 2015，samples of 68 breast cancer tissues，40 adjacent normal breast tissues，and 40 breast fibroadenoma tissues were collected. The expression of SMG 1 in the above tissues was detected by immunohistochemistry SP method. Real-time quantitative PCR（QPCR）was applied for the detection of mTOR and Raptor levels. Correlation of SMG 1，mTOR and Raptor with clinicopathological features of breast cancer was studied. Spearman correlation analysis was used to analyze the correlation between SMG 1 expression and mTOR, Raptor expression. Results The positive expression rate of SMG 1 in breast cancer tissues was 27.9％（19／68），lower than 77.5％（31／40） of adjacent normal breast tissues and 82.5％（33／40）of breast fibroadenoma tissues（P＜0.05）. The levels of mTOR and Raptor in breast cancer were 2.754±0.213 and 4.137±0.626，higher than those of adjacent normal breast tissues and breast fibroadenoma tissues（P＜0.05）. SMG 1 expression was related to tumor size，lymph node metastasis and histological grade（P＜0.05）. mTOR was related to tumor size， TNM stage and histological grade（P＜0.05）. Raptor expression was related to tumor size，lymph node metastasis and histological grade （P＜005）. SMG 1 expression was negatively correlated with mTOR and Raptor expression（r=-0.547，r=-0.415，P＜0.05），and mTOR expression was positively correlated with Raptor expression （r=0.664，P＜0.05）. Conclusion There are high expression of mTOR and Raptor, and low expression of SMG 1 in breast cancer tissues. Expression of mTOR，Raptor and SMG 1 are correlated with tumor size and histological grade，indicating that SMG 1, mTOR and Raptor may play an important role in the occurence and development of breast cancer.