| 复方苦参含药血清体外诱导人结肠癌细胞SW480的凋亡 |
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| 引用本文： | 周林,韩石平,杨翀,金弢,梁保康. 复方苦参含药血清体外诱导人结肠癌细胞SW480的凋亡[J]. 中国中西医结合外科杂志, 2006, 12(1): 54-56 |
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| 作者姓名： | 周林 韩石平 杨翀 金弢 梁保康 |
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| 作者单位： | 杭州市红十字会医院(浙江省中西医结合医院)普外科,杭州,310003 |
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| 摘 要： | 目的:进一步探讨复方苦参含药血清对人结肠癌细胞SW480的凋亡诱导作用.方法:采用血清药理学方法研究复方苦参对结肠癌细胞SW480的体外效应.观察含药血清对SW480细胞克隆形成率的影响,应用荧光染色,流式细胞仪及透射电镜观察含药血清对SW480的凋亡诱导作用.结果:含药血清对SW480细胞克隆形成率有一定抑制作用.含药血清作用细胞48 h后,可观察到胞核固缩、荧光染色增强、胞核碎裂等凋亡形态学变化,流式细胞仪检测到亚G1峰.结论:复方苦参含药血清体外抑制SW480细胞的增殖并能诱导其凋亡,诱导凋亡是其抗肿瘤的机制之一.
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| 关 键 词： | 结肠肿瘤 血清药理学 复方苦参 凋亡 |
| 文章编号： | 1007-6948(2006)01-0054-03 |
| 收稿时间： | 2005-03-10 |
| 修稿时间： | 2005-07-12 |
Apoptosis of Human Colon Cell Line SW480 Induced by Radix Sophorae Elavescentis Compouds Preparation Contained Serum in Vitro |
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| Zhou Lin, Han Shiping, Yang Cong, et al, China). Apoptosis of Human Colon Cell Line SW480 Induced by Radix Sophorae Elavescentis Compouds Preparation Contained Serum in Vitro[J]. Chinese Journal of Surgery of Integrated Traditional and Western Medicine, 2006, 12(1): 54-56 |
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| Authors: | Zhou Lin Han Shiping Yang Cong et al China) |
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| Abstract: | Obuective To explore the effect of Radix Sophorae Flavescentis(KCP) contained serum in inducing apoptosis of human colon cell line SW480 Methods Methods of sero-pharmacology were taken in the study.The effect of KCP contained serum on cloning efficiency in SW480 were evaluated.Apoptosis of SW480 were observed by fluorescent staining, transmission electronic microscopy and flow cytometry(FCM). Results KCP contained serum could inhibit the cloning efficiency of SW480.After treated by KCP contained serum for 48 hours,the cells showed features of apoptosis and Sub-G_1 peak was detected by FCM. Conclusion In vitro,KCP contained serum can inhibit cell prolife- ration and induce apoptosis of SW480.Inducing apoptosis maybe one of the anti-neoplasm mechanism of the medicine. |
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| Keywords: | colon neoplasm sero-pharmacology radix sophorae flavescentis cell apoptosis |
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