四逆散活性成分对抑郁症大鼠慢性应激模型行为学及脑内单胺类神经递质的影响

目的　研究抑郁症慢性应激模型及氯丙咪嗪、四逆散活性成分治疗后大鼠行为学改变和前皮层、海马单胺类神经递质含量的变化。方法　采用高效液相—电化学方法分析应激及四逆散活性成分对神经递质含量的影响 ,以探讨四逆散活性成分抗抑郁的机制。结果　抑郁症慢性应激模型大鼠体重显著减轻 ,进入敞箱后排便次数增多 ,水平和垂直活动明显减少 ,蔗糖水消耗量下降 (P <0 0 1) ,海马 5 HT含量减少 (P <0 0 1) ,皮层DA含量减少 (P <0 0 5 )。四逆散活性成分能升高 5 HT、DA含量 (P <0 0 1) ,进而改善大鼠行为特征。结论　调节 5 HT、DA神经递质是四逆散活性成分抗抑郁机制之一

Effects of the Active Components of Sini Powder on the Behavior and Cerebral Monoamine Neurotransmitters in the Rat Model of Depressive Chronic Stresss

Objective To study the rat model of depressive chronic stress and to investigate the behavioral changes and the changes in the levels of monoamine neurotransmitters in the anterior cortex and hippocampus in the model rats after being treated with chlorimipramine and the active components of Sini Powder (ACSP). Methods HPLC ECD techniques were used to analyze the influences of chronic stress and ACSP on the levels of monoamine neurotransmitters in order to understand the antidepressive mechanism of ACSP. Results In the model rats, the body weight decreased markedly and the times of defecation increased in the open chest and also, there was a reduction in the level and vertical movements, the consumption of sugar water ( P <0 01), the levels of 5 HT in the hippocampus ( P <0 01) and DA in the anterior cortex ( P <0 05). ACSP could increase the levels of 5 HT and DA ( P <0 01), hence, improving the behavior of the model rats. Conclusion Regulating the levels of 5 HT and DA is one of the antidepressive mechanisms of ACSP.