胸腺基质淋巴细胞生成素及相关因子在原因不明复发性流产中的表达

目的:探讨胸腺基质淋巴细胞生成素(TSLP)与原因不明复发性流产(URSA)的关系。方法:建立研究组自然流产小鼠模型(CBA/J×DBA/2)和对照组正常妊娠小鼠模型(CBA/J×Balb/c),采用免疫印迹(Western Blot)方法和实时荧光定量聚合酶链式反应(RT-PCR)方法检测绒毛、蜕膜组织中TSLP的表达;采用酶联免疫吸附双抗体夹心(ELISA)方法检测外周血TSLP、细胞因子白介素-4(IL-4)及干扰素-γ(IFN-γ)的表达;采用流式细胞仪检测调节性T细胞(Treg)表面FoxP3的表达。结果:RT-PCR法检测自然流产模型组绒毛组织中TSLP的表达低于正常妊娠模型组(P<0.05);Western Blot法分析显示自然流产模型组绒毛、蜕膜组织中TSLP的表达低于正常妊娠模型组(P<0.05);ELISA法检测自然流产模型组血清中TSLP、IL-4的表达低于正常妊娠模型组(P<0.05),而IFN-γ的表达较正常妊娠模型组未见明显增高(P>0.05);流式细胞仪检测自然流产模型组CD4+CD25+Treg和FoxP3的表达明显低于正常妊娠模型组(P<0.05)。结论:TSLP明显降低,可能是导致URSA患者母胎界面免疫耐受异常的重要原因。

Expression of Thymic Stromal Lymphopoietin and Correlated Factors in Unexplained Recurrent Spontaneous Abortion

Objective:To investigate the relationship between thymic stromal lymphopoietin(TSLP)and unexplained recurrent spontaneous abortion(URSA)。Methods:The mouse models of spontaneous abortion (CBA/J×DBA/2)and normal pregnancy(CBA/J×Balb/c)were established.The expression of TSLP in chorion and decidual tissues was screened by RT-PCR、Western Blot.ELISA was applied to detect expression of cytokines TSLP、cytokines IL-4 and IFN-γfrom peripheral blood.The expression of FoxP3 was detected by FACs.Results:Real-time PCR analysis indicated that the expression of TSLP in chorion spontaneous abortion model group was lower than normal pregnancy model group(P<0.05).Western Blot analysis indicated that the expression of TSLP in chorion and decidua spontaneous abortion model group was lower than normal pregnancy model group(P<0.05).The expression of TSLP and IL-4 in spontaneous abortion model group was lower than in normal pregnancy model group(P<0.05),but the expression of IFN-γwas no significant change than in normal pregnancy model group(P>0.05);The expression of CD4 + CD25 + Treg and FoxP3 in spontaneous abortion model group was significant lower than in normal pregnancy model group (P<0.05).Conclusions:The obvious decrease of TSLP might be an important factor for the disturbance of fatal-maternal immune-tolerance.