survivin、FHIT及bFGF在宫颈鳞癌组织中的表达及意义

目的:研究凋亡抑制基因survivin、抑癌基因FHIT及碱性成纤维细胞生长因子(bFGF)在正常宫颈组织、宫颈上皮内瘤变组织(CIN)及宫颈鳞癌组织中的表达及其与宫颈鳞癌临床分期、病理分级及淋巴结转移的关系,探讨其在宫颈鳞癌发生、发展中的作用。方法:分析郑州大学第二附属医院病理科2008年1月至2009年11月手术切除、病理检查证实为宫颈鳞癌的组织标本45例,另取CIN45例、正常宫颈组织15例(子宫肌瘤行子宫全切除术的宫颈组织)作为对照,应用免疫组化SP法检测survivin、FHIT及bFGF在3组宫颈组织中的表达情况。结果:sur-vivin在正常宫颈组织、CIN及宫颈鳞癌组织中的阳性表达呈上升趋势(χ2=11.429,P<0.05),survivin的表达在宫颈鳞癌不同临床分期、病理分级间比较,差异有统计学意义(P<0.05);FHIT在正常宫颈组织、CIN及宫颈鳞癌组织中的阳性表达呈下降趋势(χ2=24.640,P<0.05),FHIT的表达与宫颈鳞癌病理特征的关系中,仅在淋巴结有无转移方面比较差异有统计学意义(P<0.05);bFGF在正常宫颈组织、CIN及宫颈鳞癌组织中的阳性表达依次呈上升趋势(χ2=17.552,P<0.05),bFGF的表达在宫颈鳞癌不同临床分期、病理分级及淋巴结有无转移方面比较,差异均有统计学意义(P<0.05)。结论:survivin、FHIT、bFGF的表达与宫颈病变的进展有一定的关系,并且可能与宫颈鳞癌的发生、发展及浸润和转移有关。

Expression and Significance of Survivin, FHIT and bFGF in Cervical Squamous Cancer

Objective:This paper aimed to study the expression of FHIT,survivin and bFGF in normal cervical tissue,cervical intraepithelial neoplasia and cervical squamous cancer tissue as well as the relationship between the three factors and cervical squamous cancer clinicopathologic features so as to explore their role in the development of cervical squamous cancer.Methods:The specimens were collected during surgery in the Second Affiliated Hospital of Zhengzhou University from January,2008 to November,2009.They included 45 cases of cervical squamous cancer,45 cases of cervical intraepithelial neoplasia(CIN) and 15 cases of normal cervixs.Streptavidin perorxidase immunostaining technique was used to examine the expression of survivin,FHIT and bFGF in these specimens.Rusults:The expressions of survivin increased gradually from normal cervix,CIN to cervical squamous cell carcinoma(χ2=11.429,P<0.05).Also the expression of survivin was significantly correlated with clinical stage and histological grade(P<0.05).The expression of FHIT decreased gradually from normal cervix,CIN to cervical squamous cell carcinoma(χ2=24.640,P<0.05).The expression of FHIT was significantly correlated with lymph node metastases(P<0.05).The expression of bFGF increased gradually from normal cervix,CIN to cervical squamous cell carcinoma(χ2=17.552,P<0.05).The expression of bFGF was correlated with clinical stage,pathological grade and lymph node metastases(P<0.05).Conclusions:Survivin,FHIT,bFGF have roles in the development of cervical lesions,especially in the carcinogenesis and metastases of cervical squamous cell carcinoma.