乌司他丁对创伤性脑水肿合并海水淹溺性肺水肿大鼠的治疗作用

目的 探讨乌司他丁对创伤性脑水肿(TBE)合并海水淹溺性肺水肿(PE-SWD)大鼠的治疗作用. 方法 32只SD大鼠按照随机数字表法分为对照组(8只)和治疗组(24只).脑侧方液压打击伤+气管内灌注海水建立大鼠TBE合并PE-SWD动物模型.伤后治疗组腹腔注射不同剂量(2500、50 000、100 000 U/kg)乌司他丁溶液1 mL,对照组腹腔注射1 mL生理盐水,伤后24 h观察脑、肺组织含水量变化,血清、脑组织、肺组织白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量的变化以及脑、肺组织病理学变化. 结果 乌司他丁治疗后.TBE合并PE-SWD大鼠脑、肺组织含水量及血清、脑组织、肺组织IL-1β和TNF-α含量均明显降低,差异有统计学意义(P<0.05);脑、肺组织病理学改变有明显减轻. 结论 乌司他丁可以减轻TBE及PE-SWD.其机制与抗炎作用有关.

Therapeutic effect of ulinastatin on traumatic brain edema accompanied by seawater drowning in rats

Objective To observe the therapeutic effect of ulinastatin on traumatic brain edema (TBE) accompanied by pulmonary edema due to seawater drowning in rats. Methods Thirty-two Sprague-Dawley rats were randomly divided into control group (nffi8) and ulinastatin treatment group (n=24). A rat model of moderate brain trauma was established by lateral head impact, and pulmonary edema was induced in these rats by pulmonary lavage with seawater to mimic seawater drowning. Twenty-four hours after intmperitoneal injection of ulinastatin, the changes in the cerebral and pulmonary water contents and concentrations of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) in the brain, lungs and serum were measured, and the histopathological changes of the brain and lung tissues were observed. Results The cerebral and pulmonary water contents and the IL-1β and TNF-α concentrations in the serum, brain and lungs of the rats with brain trauma and pulmonary edema were markedly decreased after ulinastatin injection, which also resulted in obvious improvement of the brain and lung pathologies induced by the injuries. Conclusion Ulinastatin can alleviate traumatic brain edema in rats with pulmonary edema due to seawater drowning by inhibiting the proinflammatory cytokines.