| 阿糖胞苷诱导肺腺癌细胞凋亡与p73激活相关 |
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| 引用本文： | 张露,刘宁,吕丹瑜,马万云,李英.阿糖胞苷诱导肺腺癌细胞凋亡与p73激活相关[J].解剖学报,2011,42(1):50-53. |
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| 作者姓名： | 张露 刘宁 吕丹瑜 马万云 李英 |
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| 作者单位： | 1. 北京大学基础医学院人体解剖学与组织学胚胎学系，100191 北京; 2. 清华大学物理系原子分子纳米科学教育部重点实验室， 北京 100191 |
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| 摘 要： | 目的 体外观察阿糖胞苷(1-β-D-arabinofuranosylcytosine, Ara-C)对肺腺癌A549细胞凋亡的诱导作用,探讨p53、p73基因在此凋亡过程中的调控作用.方法 Ara-C体外作用于A549细胞,TUNEL法检测A549细胞的凋亡;透射电镜观察A549细胞凋亡的典型超微结构;免疫印迹法检测A...
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| 关 键 词： | 阿糖胞苷 p73 p53 A549细胞 TUNEL法 透射电镜 免疫印迹法 |
| 收稿时间： | 2010-4-16 |
| 修稿时间： | 2010-5-5 |
p73 activation is involved in regulation of Ara-C-induced apoptosis in human lung adenocarcinoma cells |
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| ZHANG Lu,LIU Ning,L Dan-yü,MA Wan-yun,LI Ying.p73 activation is involved in regulation of Ara-C-induced apoptosis in human lung adenocarcinoma cells[J].Acta Anatomica Sinica,2011,42(1):50-53. |
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| Authors: | ZHANG Lu LIU Ning L Dan-yü MA Wan-yun LI Ying |
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| Institution: | 1. Department of Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100083 China;2. Key Laboratory for Atomic and Molecular Nanosciences of the Ministry of Education, Department of Physics, Tsinghua University, Beijing 100191, China |
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| Abstract: | Objective To study the apoptosis pathway of human lung adenocarcinoma cell line A549 induced by 1-β-D-arabinofuranosylcytosine (Ara-C) in vitro. Methods A549 cells were incubated with Ara-C for 72hours in vitro. Biological changes of apoptotic cells were studied by TUNEL staining. Morphological changes of the A549 cells treated with Ara-C were observed by transmission electron microscope. The expressions of p53 and p73 were investigated by Western blotting. Results 1.Apoptotic rates of A549 cells exposure to Ara-C studied by TUNEL staining were higher than that of the control (P＜0.01). 2.Apoptosis body was apparently observed by transmission electron microscope. 3.Endogenous p73 but not p53 was induced and activated in dose-dependent manner upon Ara-C treatment by Western blotting.Conclusion Ara-C can effectively induce apoptosis of A549 cells. DNA damage-induced apoptosis of A549 cells treated by Ara-C is independent of functional p53.Up-regulation of p73 may play an important role that enhances the sensitivity of A549 cells to Ara-C and be partly responsible for p53-independent apoptosis. |
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| Keywords: | p73 p53 |
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