慢性低氧钙激活氯通道在大鼠肺动脉平滑肌细胞增殖中的作用 |
| |
引用本文: | 杨朝,金立元,黄建华,朴文花,郑亚丽,张珍祥. 慢性低氧钙激活氯通道在大鼠肺动脉平滑肌细胞增殖中的作用[J]. 宁夏医学杂志, 2010, 32(7): 579-581,F0002 |
| |
作者姓名: | 杨朝 金立元 黄建华 朴文花 郑亚丽 张珍祥 |
| |
作者单位: | 1. 宁夏自治区人民医院,宁夏,银川,750021 2. 华中科技大学同济医学院附属同济医院,湖北,武汉,430030 |
| |
摘 要: | 目的探讨慢性低氧时钙激活氯通道(ClCa)在大鼠肺动脉平滑肌细胞(PASMCs)增殖中的作用。方法将PASMCs分别置于常氧及慢性低氧下,采用形态学、流式细胞术、免疫细胞化学法,观察ClCa阻滞剂尼氟灭酸(NFA)和indaryloxyacetic acid(IAA-94)对PASMCs增殖的影响。结果慢性低氧:①PASMCs呈合成表型,而常氧为收缩表型,NFA和IAA-94干预后呈合成表型的PASMCs向收缩型转变;②S+G2M期细胞所占比例增高,NFA和IAA-94干预后减低,且G0G1期细胞所占比例增加(P〈0.01);③PCNA表达阳性率增高,NFA和IAA-94干预后降低(P〈0.01);④c-fos和c-jun蛋白阳性染色A增高,NFA和IAA-94干预后降低(P〈0.01)。结论慢性低氧引起细胞表型改变,可促进细胞增殖,而抑制ClCa的活性可抑制细胞的增殖,提示ClCa参与了低氧PASMCs的增殖。
|
关 键 词: | 肺动脉平滑肌细胞 钙激活氯通道 尼氟灭酸 细胞增殖 |
Effect of calcium-activated chloride channels on proliferation of pulmonary artery smooth muscle cells in rats under chronic hypoxic condition |
| |
Affiliation: | YANG Zhao,et al.(Ningxia People's Hospital,Yinchuan 750021,China) |
| |
Abstract: | Objective To investigate the effect of calcium-activated chloride(ClCa) channels on proliferation of pulmonary artery smooth muscle cells(PASMCs) in rats under chronic hypoxic condition.Methods The cultured PASMCs were under normoxic and chronic hypoxic conditions:The cells were observed by light and electron microscope;The cell cycles were observed by flow-cytometry;Immunocytochemistry staining was used to detect the expressions of PCNA,c-fos and c-jun of PASMCs.Results The PASMCs were contractile phenotype under normoxic condition and were synthetic phenotype under chronic hypoxic condition.After NFA and IAA-94,the situations were reversal;The number of S + G2M PASMCs were significantly increased in chronic hypoxic condition;The NFA and IAA-94 were significantly decreased after intervention and the number of G0G1 PASMCs were significantly increased(P 0.01) ;In chronic hypoxic condition,the expression of proliferating cell nucleus antigen was significantly increased;The NFA and IAA-94 were significantly decreased(P 0.01) ;The expression A of c-fos and c-jun were significantly increased in chronic hypoxic condition;The NFA and IAA-94 were respectively significantly decreased(P 0.01).Conclusion Hypoxic initiated the changes of PASMCs from contractile to synthetic phenotype could increase proliferation of PASMCs.NFA and IAA-94 could depress cell proliferation by blocking ClCa channels in hypoxic condition.These may play an important role in proliferation of PASMCs under chronic hypoxic condition. |
| |
Keywords: | Pulmonary artery smooth muscle cells Ca2+-activated Cl-channels Niflumic acid Cell proliferation |
本文献已被 维普 万方数据 等数据库收录! |
|