酶解泥鳅蛋白免疫调节活性的研究

根据推荐的人群日摄入量（0．02g.kg^-1）扩大5、10、20倍，剂量分别为0．1、0．2、0．4g.kg^-1，给昆明种小鼠连续灌胃酶解泥鳅蛋白一个月后，分别采用常规方法测试小鼠的各种免疫指标，观察酶解泥鳅蛋白对小鼠的免疫调节活性。结果表明：与空白组比较，低、中、高剂量组明显增强ConA诱导的小鼠脾淋巴细胞增殖能力、二硝基氟苯诱导的小迟发型变态反应、小鼠腹腔巨噬细胞吞噬鸡红细胞功能、抗体生成细胞能力和碳粒廓清能力，并能够显著升高小鼠血清血素含量。按照免疫调节作用评价程序规定，酶解泥鳅蛋白具有免疫调节作用。

Study on Immunonodulating Activities of Enzymolyzed Loach Protein

The immunomodulating activities of enzymolyzed loach protein in mice were investigated by regular methods for immunology evaluation. The doses of enzymolyzed loach protein were 0.1, 0.2, 0.4 g.kg-1 bw, which were 5, 10, 20 times respectively as large as the recommended daily intake(0.02 g.kg-1 bw). After being successively fed with enzymolyzed loach protein at given doses for 30 days by ig respectively, the mice were detected by immunomodulation tests. The results indicated that enzymolyzed loach protein had significant modulating effects on all of test indexes and suggested it had apparent immunomodulating activities in mice. Based on the results described above, we speculate that the enzymolyzed loach protein may have immunomodulating functions and can enhance the immunity of body.