体外诱导蓝氏贾第鞭毛虫滋养体的成囊和脱囊

目的 在体外诱导蓝氏贾第鞭毛虫C2株完成其生活周期。方法 通过提高改良TY-S-33培养基中的胆汁浓度至10mg／ml、pH至7．8来诱导成囊；体外诱导的包囊先经酸刺激，再用胰蛋白酶处理而诱导其脱囊。结果 体外成功诱导了蓝氏贾第鞭毛虫的成囊和脱囊。体外诱导的包囊呈椭圆形，光镜下观察有折光性，经诱导后，部分脱囊或完全脱囊成滋养体，形成的滋养体接近于梨形。结论 通过诱导，蓝贾第鞭毛虫C2株可在体外完成其生活史。

In Vitro Encystation and Excystation of Giardia lamblia Trophozoites

Objective To induce Giardia lamblia C2 strain to complete its life cycle in vitro. Methods Encystation was completed by increasing the concentrati on of bovine bile to 10mg/ml and pH to 7.8 in the modified TYI-S-33 medium. Ex cystation was induced by the two-step method. Firstly the in vitro-derived cys ts were treated by a low-pH solution and subsequently by trypsin. Results The l ife cycle of Giardia lamblia C2 strain were successfully induced to complete in vitro. In vitro-derived cysts were oval and refractive and could be induced to excystion. Partially excysted and totally excysted trophozoites were looked like a pear. Conclusions The life cycle of Giardia lamblia of C2 strain could be ind uced to complete in vitro.