Mucosal immunization with attenuated Shigella flexneri harboring an influenza hemagglutinin DNA vaccine protects mice against a lethal influenza challenge |
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Authors: | Vecino William H Quanquin Natalie M Martinez-Sobrido Luis Fernandez-Sesma Ana García-Sastre Adolfo Jacobs William R Fennelly Glenn J |
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Affiliation: | Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA. |
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Abstract: | Mucosal surfaces are important for the induction of immunity against influenza virus. In a murine intranasal immunization model, we demonstrated that the attenuated Shigella flexneri Deltaasd strain 15D, carrying a DNA construct encoding the influenza virus hemagglutinin (HA), induces protective immunity against a lethal respiratory challenge with influenza A/WSN/33. Influenza virus-specific IFN-gamma T cells were detected among splenocytes, and anti-HA IgG was detected in serum post-immunization, albeit at low levels. Following influenza virus challenge, an accelerated anti-HA IgA antibody response was detected in bronchoalveolar lavage (BAL) washings from mice vaccinated with attenuated shigella containing the HA construct. These results suggest that S. flexneri Deltaasd strain 15D is a promising vector for mucosal DNA vaccine immunization against influenza virus and other mucosal pathogens. |
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Keywords: | Influenza virus Hemagglutinin Attenuated shigella Bacterial vector DNA vaccines IFN-γ Mucosal immunity Pandemic T cell |
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