过钒酸钠对电离辐射后BET-2细胞周期阻滞及细胞凋亡的影响

  目的 观察酪氨酸磷酸酶（PTP）特异性抑制剂过钒酸钠（Per）对电离辐射诱导造血细胞周期阻滞及细胞凋亡的影响。方法 BET-2细胞经1，3，5，7，10 Gy 60COγ射线照射，用流式细胞仪观察Per对电离辐射后细胞周期改变以及细胞凋亡的影响。结果 电离辐射后，BET-2细胞出现G2／M期阻滞以及细胞凋亡，具有一定的时间效应和剂量效应；而加入Per可进一步增加G2／M期阻滞，显著减少细胞凋亡。结论 过钒酸钠可进一步增加辐射诱导的细胞周期阻滞，减少细胞凋亡，为寻找治疗辐射损伤药物提供新的途径。

Effects of pervanadate on radiation-induced G2/M arrest and apoptosis in BET-2 cells

Objective To study the effects of Pervanadate（Per） on BET-2 cells cell cycle arrest and apoptosis induced by ionizing radiation. Methods The BET-2 cells were radiated with 0, 1, 3, 5, 7, 10 Gy and divided into PTP(protein tyrosine phosphatases)-specific inhibitor Per-treated group and non-pervanadate group(served as a control) and then incubated after γ-ray irradiation. Cell cycle and apoptosis were measured by FCM and Hematoxylin-Eosin staining at different time phases. Results G2/M phase arrest and apoptosis were induced in a pattern of dose-effect and time-course after irradiation in the BET-2 cells. In Per-treated group, G2/M phase arrest increased more notably, and apoptosis decreased markedly. Conclusions These studies suggested that Pervanadate potentialy enhance the ratio and prolong the term of G2/M phase arrest, and facilitate the damaged hematopoietic cells to be repaired, remarkably prevent radiated-cells from apoptosis.