癫痫大鼠海马缝隙连接蛋白43的表达及生胃酮的保护作用

目的研究癫痫大鼠海马星形胶质细胞缝隙连接蛋白43(Connexin43,CX43)表达及缝隙连接蛋白阻滞剂生胃酮对其表达的影响。方法用免疫组化法检测癫痫发作后各时间点大鼠海马星形胶质细胞CX43免疫反应阳性表达。同时观察致痫前给予不同剂量生胃酮对大鼠海马星形胶质细胞CX43免疫反应阳性表达的影响。结果对照组大鼠海马星形胶质细胞CX43可见少量散在表达。癫痫组大鼠海马星形胶质细胞CX43表达1h即可见增加,并随时间延长而增加,72h达高峰。生胃酮各组大鼠海马星形胶质细胞CX43免疫反应阳性表达较同一时间点癫痫组低(P<0.01),且与生胃酮剂量呈负相关(P<0.01)。结论癫痫大鼠海马缝隙连接蛋白43的表达与癫痫发病机制密切相关。缝隙连接蛋白阻滞剂生胃酮影响缝隙连接蛋白43表达,具有肯定的神经保护作用。

The expression of connexin43 within the hippocampus of the epilepsy rats and the protective effects of carbenoxolone

Objective To study the relationship between the expression of connexin43 within the hippocampus astrocyte of the adult rats and pathogenesis of epilepsy and the influence of carbenoxolone on the expression of CX43 as gap junction antagonist.Methods The expression of CX43 in different time points in hippocampus astrocytes of rat brain was investigated by immunohistochemistry.Meanwhile,the effects of different dose of carbenoxolone on the expression of CX43 in hippocampus astrocytes of rat brain administered before epilepsy was evaluated.Results The basic sporadic expression of CX43 in hippocampus astrocytes could be observed in control group rats.The expression of CX43 could be observed in the epilepsy group rats 1h after epilepsy,and increased gradually,reached peak in 72 hours.The expression of CX43 in hippocampus astrocytes of rat brain in each carbenoxolone group was less(P<0.01)than that in the epilepsy group rats(P<0.01),and was downregulated as the dose of carbenoxolone increased(P<0.01).Conclusion There is strong correlation between the expression of connexin43 in hippocampus astrocyte of epilepsy rats brain and the the pathogenesis of epilepsy.The gap junction antagonist carbenoxolone affected the expression of connexin43 in rat brain,and plays positively neuroprotective effects.