几种重金属化合物对血管内皮细胞的毒性研究

目的探讨几种重金属化合物对血管内皮细胞的毒性作用。方法以人脐静脉内皮细胞株CRL-2480为研究对象,与不同浓度(0、0.05、0.1、0.5、1、5、10、20μmol/L)氯化锰(MnCl2)、氯化镉(CdCl2)、乙酸铅[(C2H3O2)2Pb]、氯化镍(NiCl2)、氯化铬(CrCl3)或三氧化铬(CrO3)分别孵育12、24、487、2 h后,MTT法检测细胞活性。此外,荧光法检测氯化镉孵育24 h对内皮细胞骨架F-actin的影响。结果除氯化镍外,其他几种重金属化合物在0.05～20μmol/L的作用浓度下对血管内皮细胞具有不同程度的毒性作用,并呈时间及剂量依赖性;其中以+6价铬(Ⅵ)及镉对细胞毒性最强。氯化镉可引起细胞骨架重排、破坏。结论锰、镉、铅、铬(Ⅲ)及铬(Ⅵ)均对血管内皮细胞具有毒性作用,长期摄入重金属污染的水产品可能与心血管疾病的发生发展有关。

The toxic effects of heavy metals on the endothelial cells

Objective To investigate the toxic effects of several heavy metals on the vascular endothelial cells.Methods Human umbilical vein endothelial cells(CRL-2480) were treated with MnCl2,CdCl2,(C2H3O2)2Pb,NiCl2,CrCl3 or CrO3 at various concentrations(0,0.05,0.1,0.5,1,5,10,and 20 μmol/L).MTT assay was carried out to determine the cell viability.In addition,after treatment with various concentrations of CdCl2 for 24 h,the F-actin in cells was observed with fluorescence microscope.Results Except for NiCl2,the other heavy metal chemicals resulted in time-and concentration-dependent injury for cell viability.Endothelial cell cytoskeleton was disorganized after treatment with CdCl2 0.05~20 μmol/L.Conclusion Mn,Cd,Pb,Cr(Ⅲ) and Cr(Ⅵ) are toxic to endothelial cells.Chronic absorption of heavy metal chemicals may contribute to development cardiovascular diseases.