微卫星用于鸡群体遗传结构研究

目的 探索微卫星用于研究实验动物群体遗传结构的可行性。方法 用选自鸡基因组中的20个微卫星标记，对岭南黄鸡100个个体的基因组DNA进行PCR扩增，聚丙烯酰胺电泳后，检测微卫星多态性，并检测出多态位点的等位基因数、平均多态信息含量(PIC)和平均杂合度(H)。结果 在20个微卫星中，有13个微卫星呈现出明显的多态性，其等位基因数为4～7个，平均多态信息含量(PIC)为0．6109，平均杂合度(H)为0．67l2。结论 微卫星是一种研究实验动物群体遗传结构的有效方法。

Microsatellite Applied in Study of Genetic Structure in Chicken Population

Twenty microsatellite DNA markers were selected from poultry genome in this study. The genomes of 100 Lingnan yellow chickens were analysed. the results from this study indicated that 13 of 20 microsatellite DNA markers were polymorphic.The number of alleles was 4-7, the average value of polymorphism information content was 0^6109 and the average value of heterozygosity was 0^6712.;