海风藤提取物对脑缺血再灌注模型大鼠神经细胞凋亡和线粒体膜电位变化的干预作用

目的观察脑缺血再灌注2h后大鼠神经细胞凋亡和线粒体膜电位的变化及海风藤提取物的干预作用。方法应用线栓法建立大鼠大脑中动脉阻塞再灌注模型,流式细胞术分别观察假手术组、模型组、二甲基亚砜组和海风藤组缺血灶周神经细胞凋亡和线粒体膜电位的变化。结果与模型组神经细胞凋亡率(72.7%±6.5%)和线粒体膜电位(0.117±0.011)比较,海风藤组神经细胞凋亡率(47.0%±3.8%)明显下降(P<0.01),线粒体膜电位(0.182±0.023)明显升高(P<0.01);而假手术组神经细胞凋亡率(5.8%±1.8%)和线粒体膜电位(0.331±0.016)与模型组比较差异均有显著性(P<0.01)。结论海风藤可以减轻脑缺血再灌注后早期神经细胞凋亡,并对线粒体膜电位有稳定作用,可以保护缺血脑组织。

Detection of the Apoptosis and Mitochondrial Membrane Potential of Nerve Cell After Cerebral Ischemia-Reperfusion and Intervention of Extraction from Piper Kadsura Ohwi

Aim To investigate the apoptosis and mitochondrial membrane potential (MMP) of nerve cell after cerebral ischemia-reperfusion for 2 hours and intervention of extraction from Piper Kadsura Ohwi. Methods The models of middle cerebral artery occlusion (MCAO) and reperfusion were set up. The apoptosis and MMP of nerve cell were detected among sham operated group, ischemic group, DMSO-treated (dimethyl sulfoxide) group and extraction from Piper Kadsura Ohwi-treated group in the cerebral tissue around infarction 2 hours after ischemia-reperfusion separately through flow cytometry. Results 2 hours after ischemia-reperfusion, the apoptosis rate of nerve cell of Piper Kadsura Ohwi-treated group (47.0%±3.8%) decreased obviously accompanied with obvious increase of MMP (0.182±0.023) compared with ischemic group (P<0.01). The apoptosis rate of nerve cell (5.8%±1.8%) and MMP (0.331±0.016) of sham operated group were different from those of ischemic group. Conclusion Extraction from Piper Kadsura Ohwi plays a significant role in protecting cerebral tissue through decreasing the apoptosis and stopping decrease of MMP of nerve cell caused by ischemia-reperfusion.