Duration of the mitotic cycle of chinese hamster cells cultured at 30–39°C

The duration of the mitotic cycle (T) and of its stages at temperatures of 30, 33, 36, and 39°C was studied by an autoradiographic method in Chinese hamster cells of subline 237. The duration of the mitotic cycle was shortest at 39°C and increased as the cultivation temperature fell. Within the temperature range 33–39°C the increase in duration of the mitotic cycle and its stages was proportional to the temperature studied. The slope of the curve of duration of the mitotic cycle as a function of temperature increased sharply as the temperature changed from 33 to 30°C. The G₁ period was most sensitive and the G₂ period least to a change in the cultivation temperature.Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 82, No. 11, pp. 1365–1367, November, 1976.