Elevated levels of homologous DNA recombination activity in the regenerating rat liver |
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Authors: | Bhaskar Thyagarajan Jennifer L. Cruise Colin Campbell |
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Affiliation: | (1) Department of Pharmacology, University of Minnesota Medical School, 3-249 Millard Hall, 435 Delaware Street, S.E., 55455 Minneapolis, Minnesota;(2) Department of Biology, University of St. Thomas, 2115 Summit Ave., 55105 St. Paul, Minnesota |
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Abstract: | We have characterized homologous DNA recombination activity in nuclear protein extracts prepared from quiescent and regenerating rat livers. Activity measured in regenerating liver extracts was elevated approximately 35-fold above control, and its appearance closely mirrored the first wave of DNA synthesis, peaking 24 hours after a regenerative stimulus, and returning fairly rapidly to basal levels. We also identified a strand-transferase protein of approximately 100 kDa whose presence in these extracts correlates with homologous recombination activity. Recent evidence suggests that mammalian somatic cells possess a recombinational DNA repair mechanism analogous to that described in the yeastSaccharomyces cerevisiae. Our results indicate that this recombinational repair process may be regulated in vivo by, or play a role in, progression through the cell division cycle. |
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