| HPLC同时测定人血清中4种非苯二氮苯卓类镇静催眠药物 |
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| 引用本文： | 莫斌斌,张毕奎,孙银香,王峰,李焕德,冯军,毛名扬. HPLC同时测定人血清中4种非苯二氮苯卓类镇静催眠药物[J]. 中国药学杂志, 2003, 38(7): 537-539 |
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| 作者姓名： | 莫斌斌 张毕奎 孙银香 王峰 李焕德 冯军 毛名扬 |
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| 作者单位： | 1.中南大学湘雅二医院临床药学研究室，湖南 长沙 410011;2.湘潭市江南机器厂职工医院，湖南 湘潭 411207;3.湖南江华县人民医院药剂科，湖南 永州 425500;4.安徽省铜陵市人民医院，安徽 铜陵 244000 |
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| 摘 要： | 目的建立一种同时测定多种非苯二氮类镇静催眠药物的方法，用于临床中毒的定性与定量分析。方法血样用乙醚一步萃取，二极管阵列检测器检测，波长为254nm，采用外标法，对血样中的佐匹克隆、扎莱普隆、唑吡坦、甲喹酮进行定性与定量分析。结果血浆中0.1～2 μg·mL-1浓度范围内具有良好的线性关系，血浆回收率均大于90%，日内、日间RSD均小于10%。结论本方法简单、灵敏、准确、干扰小，适用于低浓度生物样本的测定。
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| 关 键 词： | 高效液相色谱 佐匹克隆 扎莱普隆 唑吡坦 甲喹酮 |
| 收稿时间： | 2002-05-13; |
Simultaneous determination of four none benzodiazepines in human serum by means of HPLC |
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| MO Bin-bin,ZHANG Bi-kui,SUN Yin-xiang,WANG Feng,LI Huan-de,FENG Jun,MAO Ming-yang. Simultaneous determination of four none benzodiazepines in human serum by means of HPLC[J]. Chinese Pharmaceutical Journal, 2003, 38(7): 537-539 |
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| Authors: | MO Bin-bin ZHANG Bi-kui SUN Yin-xiang WANG Feng LI Huan-de FENG Jun MAO Ming-yang |
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| Affiliation: | 1.Clinical Pharmacy Research Laboratory,The Second Xiangya Hospital of Central South University,Changsha 410011,China；2.Affiliated Hospital of Jiangnan Mechanical Factory,Xiangtan 411207,China；3.People′s Hospital of Jianghua,Yongzhou 425500,China；4.People′s Hospital of Tongling,Anhui Province,Tongling 244000,China |
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| Abstract: | OBJECTIVE To establish a method to simultaneously determine various ataractic hypnagogues known as none benzodiazepines for clinical qualitative and quantitative analysis. METHODS Blood samples were extracted by ether and tested with diode detector,with the detection wavelength of 254 nm.Qualitative and quantitative analysis was carried out with external standardization. RESULTS Good linear relation was obtained over the range from 0.1 to 2μg·mL-1.The average recovery of the assay exceeded 90 percent.And the within day and between day RSD was less than 10 percent. CONCLUSION This method is simple,ingenious and accurate with little interference.It is applicable to the determination of some biological samples in low concentrations. |
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| Keywords: | HPLC zopiclone zaleplon zolpidem methaqualone |
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