He-Ne激光照射对豚鼠表皮Langerhans细胞的影响

目的 观察不同剂量Ｈｅ－Ｎｅ激光照射对Ｌａｎｇｅｒｈａｎｓ细胞产生的影响。方法 ５６只Ｄｕｎｋｉｎ－Ｈａｒｔｌｅｙ雄性白色豚鼠分７组，每组８只。６组为激光照射组，分别给予１．１６、２．３２、４．６５Ｊ／ｃｍ＾２的Ｈｅ－Ｎｅ激光照射（各２组），每日１次；另１组为对照组。连续照射７天后，第１、３天麻醉下活体取豚鼠照射区皮肤，采用ＡＴＰ酶染色法测定ＡＴＰ酶阳性细胞（Ｌａｎｇｅｒｈａｎｓ细胞）密度。结果 ２．３２Ｊ／ｃｍ＾２的Ｈｅ－Ｎｅ激光照射后第１、３天组和４．６５Ｊ／ｃｍ＾２的Ｈｅ－Ｎｅ激光照射后第１天组的豚鼠，其表皮ＡＴＰ酶阳性细胞密度均高于对照组，差异均有非常显著意义（Ｐ〈０．０１）。结论 一定剂量的Ｈｅ－Ｎｅ激光照射可增加豚鼠表皮Ｌａｎｇｅｒｈａｎｓ细胞的数量，其数量的增加可能影响Ｌａｎｇｅｒｈａｎｓ细胞的抗

Effect of He-Ne Laser Irradiation on Epidermal Langerhans Cells in Guinea Pigs

Objective To purpose of this study was to investigate whether low energy He-Ne laser irradiation affects Langerhans cells in the epidermis. Methods 56 healthy male guinea pigs were randomly divided into 7 groups, 6 irradiation groups and 1 control group, 8 animals each. The skin on the back of guinea pigs was irradiated by He-Ne laser with an output power of 19 mW, spot size 2.5 cm in diameter, 5, 10 and 20 minutes(energy density of 1.16, 2.32, 4.65 J/cm 2), respectively, per day for consecutive 7 days. One and 3 days after completion of laser irradiation. the animals were killed and the taken skin samples were stained using ATP-staining technique. The numbers of ATPase positive cells(Langerhans cells) present in the epidermis were calculated under microscope and compared with that of control group. Results Laser irradiation with an energy density of 2 32 J/cm 2 and 4 65 J/cm 2 increased the number of Langerhans cells in the epidermis significantly, and the dose of 2 32 J/cm 2 was most effective. Conclusions Our findings indicate that a certain dose of low energy He-Ne laser irradiation may increase the number of epidermal Langerhans cells, which might be favorable to improve their antigen-presenting function.