安体舒通对单侧输尿管结扎大鼠肾脏的抗氧化保护作用

目的:探讨醛固酮受体拮抗剂安体舒通对大鼠单侧输尿管梗阻(UUO)模型肾间质纤维化的抗氧化保护作用.方法:Wistar 大鼠行左侧输尿管结扎术,分为UUO模型组(n=11)和安体舒通治疗组(n=12),同时设假手术对照组(n=7).术后第14天处死各组大鼠,进行HE和Masson染色,观察肾脏病理变化;比色法测定肾组织丙二醛(MDA)和超氧化物歧化酶(SOD)含量;放免法测定血浆和组织醛固酮(ALD)含量.结果:UUO模型组与假手术组比较,肾脏病理改变加重,肾组织MDA含量明显升高,SOD含量显著下降,血浆和组织ALD含量明显升高(P＜0.05).安体舒通组与UUO组大鼠比较,肾间质纤维化显著减轻,肾组织MDA含量明显降低,SOD含量显著升高,血浆和组织ALD含量下降(P＜0.05).结论:安体舒通可能减少单侧输尿管梗阻肾组织脂质过氧化物的产生,同时增加抗氧化酶的含量,从而改善UUO大鼠氧化应激所致的肾间质纤维化.

Antioxiative effects of spironolactone on rat kidney with unilateral ureteral obstruction

Objective:To investigate the antioxiative effects of spironolactone,an aldosterone receptor blockade,on kidney of rats with unilateral ureteral obstruction(UUO).Methods:Thirty wistar rats underwent UUO and then received vehicle(n = 11)or spironolactone(50mg.kg-1.d-1 by daily gastric gavage,n = 12)during the 15 days from the day before surgery.Additional 7 rats were sham operated.All the rats were killed at 14 days after surgery.Histological changes were observed by HE and Masson staining.Malondialdehyde(MDA),superoxide dismutase(SOD)as well as aldosterone(ALD)content were measured.Results:UUO induced a significant increase in MDA and ALD content but a marked decrease in SOD content and severe morphology changes.However,spironolactone ameliorated what had mentioned.Conclusions:Spironolactone can suppress oxidative stress and alleviate renal in rats with UUO by reducing lipid peroxidation production and improving antioxidative enzyme contents.