Necrosis of infectious pancreatic necrosis virus (IPNV) infected cells rarely is preceded by apoptosis |
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Authors: | Espinoza Juan Carlos Cortés-Gutierrez Marcia Kuznar Juan |
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Affiliation: | Laboratorio de Bioquímica y Virología, Instituto de Ciencias Biológicas y Químicas, Facultad de Ciencias, Casilla 50 30, Universidad de Valparaíso, Valparaíso, Chile. |
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Abstract: | Infectious pancreatic necrosis virus (IPNV)-infected cells were labeled with Annexin V and propidium iodide in order to determine the proportion of cells, which developed necrosis and/or apoptosis during the time course of infection. Contrasting with earlier reports, we found that at any time during IPNV multiplication cycle, the percentage of apoptotic cells never exceeded the 12% of the whole population of the infected cells. In addition, the percentage of necrotic cells increased continuously until reaching the 75% of the infected cells at 15 h post infection. Apoptotic cells were also identified by in situ terminal deoxynucleotidyl transferase-mediated BrdUTP nick end labeling (TUNEL). Our results are in accordance with the idea that apoptosis rarely precedes necrosis. |
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