小儿与老年白内障晶体上皮细胞密度及增殖细胞核抗原表达

目的 检测儿童和老年人晶体上皮细胞密度和增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇ ｃｅｌｌ ｎｕｃｌｅａｒ ａｎｔｉｇｅｎ,ＰＣＮＡ）表明,探讨晶体上皮细胞与白内障术拍后囊混浊的关系。方法 用ＨＥ染色观察白内障小儿组（〈１２岁）及老年组（５１￣８０岁）喘区晶体上皮铺片的细胞密度,用免疫组化染色及真彩色医学图像分析系统检测ＰＣＮＡ的表达及积分吸光度（Ａ）值。结果 晶体上皮细胞密度在小儿组为５０２０

Cell density and expression of proliferating cell nuclear antigen (PCNA) in the lens epithelium of children and aging patients with cataract

OBJECTIVE: To determine the cell density and expression of proliferating cell nuclear antigen (PCNA) in the lens epithelium of children and aging patients with cataract. METHODS: The lens epithelial cells (LECs) of the central area were examined in two groups, children (less than 12 years old) and senile persons (51 - 80 years old). The densities of LECs in the two groups were calculated with samples stained with hematoxylin and eosin (HE) staining, and the PCNA was tested immunohistochemically. The integral optical density (IOD) of PCNA expression was measured through a medical color image analysis system. RESULTS: The densities of LECs in children and senile persons were 5,020.25 +/- 246.01 cells/mm(2) and 4,340.00 +/- 240.95 cells/mm(2) (P < 0.05), the IODs were 2.89 +/- 0.57 and 2.13 +/- 0.63 (P < 0.05) respectively. CONCLUSION: The LEC counting and IOD values in children eyes are significantly higher than that of senile persons, suggesting that they be one of the important factors in the formation of posterior capsular opacification (PCO) or after cataract in children eyes.