| 人原代晶体上皮细胞bFGF多肽和mRNA的表达 |
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| 作者姓名： | Liu D Wu J |
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| 作者单位： | [1]北京市眼科研究所 [2]北京医科大学第一医院眼科 |
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| 摘 要： | 目的 为研究后发性白内障的发生机理,检测生长中人晶体上皮细胞（ＨＬＥＣｓ）自身是否表达碱性成纤维细胞生长因子（ｂＦＧＦ）。方法 体外培养人晶体上皮细胞,用免疫细胞化学和原位核酸分子杂交的方法,检测ＨＬＥＣｓ中ｂＦＧＦ多肽和其ｍＲＮＡ的表达。结果 用免疫细胞化学和原位核酸分子杂交方法,可检测到生长中的人晶体上皮细胞自身表达碱性成纤维细胞生长因子。结论 结合碱性成纤维细胞生长因子促进人晶体上皮细胞生长
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| 关 键 词： | 晶体 白内障 上皮细胞 bFGF mRNA |
Expression of bFGF in primary human lens epithelial cells |
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| Liu D,Wu J.Expression of bFGF in primary human lens epithelial cells[J].Chinese Journal of Ophthalmology,1998,34(3):205-207,I013. |
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| Authors: | Liu D Wu J |
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| Institution: | Department of Ophthalmology, First Hospital, Beijing Medical University, Beijing 100034. |
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| Abstract: | Objective To study the mechanism of after cataract formation and investigate the expression of basic fibroblast growth factor(bFGF) in primary human lens epithelial cells(PHLECs). Methods Human lens epithelial cells were cultured in vitro, and the expression of bFGF was detected with immunohistochemistry and in situ hybridization.Results PHLECs express bFGF in protein and mRNA levels.Conclusion Based on bFGF promoting PHLEC growth and differentiation,the results suggest that bFGF from PHLECs take part in promoting such cell growth and differentiation. |
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| Keywords: | Lens Cataract Epithelial cells Growth factor |
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