角质细胞生长因子促进角膜上皮损伤修复的研究

目的寻找促进角膜上皮损伤修复，治疗持续性角膜上皮缺损的有效方法。方法用３Ｈ－胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入及液体闪烁技术，观察不同浓度的外源性角质细胞生长因子（ｋｅｒａｔｉｎｏｃｙｔｅｇｒｏｗｔｈｆａｃｔｏｒ，ＫＧＦ）对体外培养的人角膜上皮细胞生长的影响，由此推算出有效滴眼液浓度并应用于兔眼。用计算机图形分析系统计算角膜上皮愈合速率；用光镜和电镜评估愈合的质量。结果０．１～１００ｎｇ／ｍｌＫＧＦ有明显促进体外培养的人角膜上皮细胞生长的作用（增长率为２７．６６％～７６．７３％），且呈剂量依赖性（ｒ＝０．９２３３，Ｐ＜０．００１）。１μｇ／ｍｌＫＧＦ滴兔眼，加速了角膜上皮损伤修复（愈合速率，ＫＧＦ组为１．７７±０．２３ｍｍ２／ｈ，与对照组１．４９±０．２４ｍｍ２／ｈ比较，Ｐ＜０．０５）。结论外源性ＫＧＦ对体外培养的人角膜上皮细胞有明显的促生长作用，其滴眼液有加速兔眼角膜上皮创伤修复的作用。

Effect of keratinocyte growth factor on corneal epithelial wound healing

OBJECTIVE: To select effective methods for accelerating corneal epithelial wound healing and treating persistent corneal epithelial defect. METHODS: Exogenous keratinocyte growth factor (KGF, 0.1 ng/ml - 100 ng/ml) was added to cultures of human corneal epithelial cells and DNA synthesis in corneal epithelial cells was measured with (3)H]-thymidine incorporation and liquid scintillation techniques. The effective concentration of topical application of KGF was calculated and 1 microg/ml KGF was then applied topically to rabbit eyes. Corneal epithelial defect was measured by a digital computer at different times. RESULTS: KGF at a dosage ranging from 1 ng/ml to 100 ng/ml enhanced cellular proliferation effectively (the rate of cell proliferation 27.66% - 76.73%) and in a dose-dependent manner (r = 0.9233, P < 0.001). Topical application of KGF significantly accelerated corneal wound healing in rabbits (Epithelial healing rate in the KGF-treated group was 1.77 +/- 0.23 mm(2)/h, while in the PBS-treated group it was 1.49 +/- 0.24 mm(2)/h; P < 0.05). CONCLUSION: The results suggest that exogenous KGF stimulate proliferation of human corneal epithelial cells and accelerate corneal epithelial wound healing in vivo.