紫外线对培养兔晶体的氧化损伤及超微形态学研究

目的 探讨实验性兔晶体氧自由基损伤与白内障的发病机制。方法 利用近距离紫外线（ＵＶ）照射体外培养兔晶体,诱发氧自由基损伤。并同时观察超氧化物歧化酶（ＳＯＤ）的抗氧化作用。实验分为ＵＶ照射组、ＵＶ＋ＳＯＤ组及对照组,采用硫代巴比妥酸比色法分别检测三组晶体匀浆中脂质过氧化产物丙二醛含量,并通过透射电镜观察三组晶体上皮细胞的超微结构。结果 ＵＶ照射组兔晶体匀浆中的丙二醛含量明显增高。ＵＶ＋ＳＯＤ组（ＵＶ

A study of lipid peroxide-induced damage and of ultrastructure of lens epithelial cells in lens organ culture in vitro

Objective To understand the mechanism of the rabbit lens damage from lipid peroxidation (LPO) involved in cataract development. Methods Ultraviolet (UV) radiation was used to induce the damage of peroxidation on cultured rabbit lens. Cultured rabbit lenses were exposed to UV radiation in MEM cultural medium without or with superoxide dismutase (SOD) added at 10 U/ml. The thiobarbituric acid (TBA) colorimetric method was used to detect the lens malondialdehyde (MDA) content produced by LPO, and electron microscopy was used to observe the ultrastructure of rabbit lens epithelium. Results In the lens with UV radiation, MDA was increased as compared with that in non irradiated one ( P <0.01) and in UV SOD group ( P <0.01). The membranes of lens epithelial cells were damaged and mitochondria, etc. membranous structures disappeared with clumping pattern of chromatin, while the cells in UV SOD and control groups appeared normal in structure. Conclusion UV radiation can induce lipid peroxide damage in lens culture of rabbit.