反相高效液相色谱法测定人血清中西洛他唑质量浓度

目的建立测定人血清中西洛他唑质量浓度的反相高效液相色谱（RP—HPLC）法。方法采用液-液萃取高效液相色谱紫外检测法，以地西泮为内标，色谱柱为Phenomenex Synergi 4u Fusion—RP C18柱（250mm×4．6mm，5μm），流动相为乙腈-水（45：55），流速1．0mL／min，检测波长258nm。结果血清中内源性物质不干扰西洛他唑的检测，西洛他唑质量浓度的线性范围为0．016～2．000μg／mL，日内、日间精密度的RSD均小于15％，低、中、高质量浓度（0．031，0．250，1．000μg／mL）溶液的平均提取回收率分别为76．8％，78．5％，77．1％。结论RP—HPLC法快速、简单、准确，符合生物样品检测要求，可用于临床药代动力学研究。

Determination of Cilostazol in Human Serum by RIP-HPLC

Objective To develop a rapid, simple and specific HPLC method for determination of cilostazol in human serum. Methods RP- HPLC was used. The analyte and diazapam, used as the internal standard, were analyzed on the Phenomenex Synergi 4u Fusion- RP C18 （250 mm × 4.6 mm, 5 μm） column at the wavelength of 258 nm after liquid - liquid extraction. Acetonitrile - water （45 ： 55, V/ V） was used as the isocratic mobile phase at a flow rate of 1.0 mL/min. Results The assay was linear over the concentration range of 0.016- 2.000 μg/mL excluded from endogenous substance effect. RSD for the intra- day and inter- day precision over the entire concentration range was less than 15%. The mean extraction recovery rates of cilostazol were 76. 8%, 78.5%, 77.1% at the concentrations of 0. 031, 0. 250, 1. 000 μg/mL, respectively. Conclusion This RP-HPLC method is simple, rapid and specific to determine cilostazol in human serum for clinical pharmacokinetics.