Isolation and identification of Malassezia species from Chinese and Korean patients with seborrheic dermatitis and in vitro studies on their bioactivity on sebaceous lipids and IL‐8 production |
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| Authors: | Soo Young Kim Se Hyun Kim Su Na Kim Ah‐Reum Kim Yu Ri Kim Min Jung Kim Won‐Seok Park John Hwan Lee Won Hee Jung Yang Won Lee Yong Beom Choe Kyu Joong Ahn |
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| Affiliation: | 1. Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea;2. AMOREPACIFIC Corp. R&D Unit, Yongin, Korea;3. Department of Systems Biotechnology, Chung‐Ang University, Anseong, Korea;4. Research Institute of Medical Science, Konkuk University, Seoul, Korea |
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| Abstract: | We investigated the distribution of Malassezia yeast in 120 Chinese (20 patients from each of six cities) and 20 Korean patients with scalp seborrheic dermatitis (SD) and dandruff (SD/D) using ITS1 and ITS2 polymerase chain reaction‐restriction fragment length polymorphism. Bioactivity was studied by quantifying sebum lipid production by human primary sebocytes and inflammatory cytokine, interleukin‐8 (IL‐8) production was studied by exposing HaCaT keratinocytes with extracts of five standard Malassezia strains; M. globosa, M. restricta, M. sympodialis, M. dermatis and M. slooffiae. M. restricta and M. globosa were the most frequently encountered species from both Chinese and Korean patients. These two Malassezia species also promoted neutral lipid synthesis although the result was not statistically significant and induced significant increase in IL‐8 production among the five Malassezia species studied. The study suggests a possible role of these organisms in the pathogenesis of SD/D. |
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| Keywords: | dandruff seborrheic dermatitis Malassezia yeast ITS1 and ITS2 PCR‐RFLP neutral lipid synthesis IL‐8 |
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