|
|||||
|
|
| B19 VP2抗原真核表达载体的构建及免疫原性观察 | |
| 引用本文： | 胡军,戚敏,张世杰,宋建伟,赵国强. B19 VP2抗原真核表达载体的构建及免疫原性观察[J]. 郑州大学学报(医学版), 2005, 40(3): 377-379 |
| 作者姓名： | 胡军 戚敏 张世杰 宋建伟 赵国强 |
| 作者单位： | 1. 郑州大学基础医学院微生物学与免疫学教研室,郑州,450052 2. 郑州大学基础医学院药理学教研室,郑州,450052 3. 郑州大学医学实验中心,郑州,450052 |
| 基金项目： | 河南省科技攻关基金资助项目01140512 |
| 摘 要： | 目的：探讨以B19 VP2基因为靶基因构建的真核表达载体作为基因疫苗的免疫原性。方法：用PCR方法以pGEM1-B19为模板扩增出B19 VP2目的基因;将其重组入真核表达载体pcDNA3;将重组子pcDNA3-VP2及空质粒分别用脂质体包裹接种家兔;ELISA方法检测家兔血清中B19 VP2抗体产生情况。结果：成功构建了真核表达载体pcDNA3-VP2;其作为基因疫苗接种家兔后可产生滴度较高,持续时间较长的抗体。结论：B19 VP2基因可作为B19病毒基因疫苗构建的靶基因。 |
| 关 键 词： | 人微小病毒B19 VP2抗原 免疫 家兔 |
| 修稿时间： | 2005-02-22 |
Construction of eukaryotic expression vector pcDNA3/VP2 and its immunological effect |
|
| HU Jun,QI Min,Zhang Shijie,Song Jianwei,ZHAO Guoqiang. Construction of eukaryotic expression vector pcDNA3/VP2 and its immunological effect[J]. Journal of Zhengzhou University: Med Sci, 2005, 40(3): 377-379 | |
| Authors: | HU Jun QI Min Zhang Shijie Song Jianwei ZHAO Guoqiang |
| Affiliation: | HU Jun 1),QI Min 2),ZHANG Shijie 1),SONG Jianwei 3),ZHAO Guoqiang 1) 1)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052 2)Department of Pharmacology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052 3)Experimental Center of Medical College,Zhengzhou University,Zhengzhou 450052 |
| Abstract: | Aim: To explore the immunogenicity of pcDNA3/VP2 as gene vaccine. Methods: B19 VP2 gene was amplified using PCR. B19 VP2 was recombined with pcDNA3 to form pcDNA3/VP2. pcDNA3/VP2 packed with liposome was inoculated into laboratory rabbit. B19 VP2 antibody was detected in rabbit's serum using ELISA.Results: Eukaryotic vector pcDNA3/VP2 was successfully constructed, which had better immunogenicity as gene vaccine and could stimulate rabbit to generate higher titre antibody. Conclusion: B19 VP2 gene can be used as target gene of B19 virus gene vaccine. |
| Keywords: | human parvovirus B19 VP2 antigen immunogenicity rabbit |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |