MMP-7在大肠癌中的表达与其侵袭转移的关系

目的探讨MMP-7在大肠癌（CRC）中的表达与其侵袭转移的关系。方法采用免疫组化SABC法及原位杂交技术检测MMP-7mRNA和其蛋白在大肠正常黏膜、腺瘤和癌组织中的表达。结果MMP-7的原住杂交及免疫组化的结果大体是-致性，MMP-7在正常黏膜中的低表达和在腺瘤、癌组织中的高表达之间的差异均有统计学意义（P〈0．001）；随着Dukes分期的升高，其表达呈上升的趋势；淋巴结和远处器官转移组的表达均高于未转移组（P〈0．05）。结论MMP-7在CRC的发生发展和侵袭转移过程中发挥着重要的作用。

Relationship between the Expresssion of MMP-7 in Colorectal Carcinoma and the Invasion and Metastasis

Objective To investigate the relationship between the invasion and metastasis of colorectal carcinoma and the expression of MMP-7. Methods The immunohistochemistry method and the in situ hybridization method were used to detect the expressions of MMP-7 in normal colorectal mucosa tissues,adenomas and colorectal carcinomas. Results The expression of MMP-7 genc detected by in situ hybridization was consistent with the MMP -7 protein expression detected by immunohistochemistry ; There was a significant difference between the high expression of MMP - 7 in colorectal carcinoma and the low expression in normal mucosa（ P 〈 0,001 ） ;The expression of MMP-7 was also significantly higher in the adenoma than that in the normal mucosa （P 〈 0. 001 ）. There was a trend toward an increased expression of MMP-7 with the progression of Dukes stages and the present of node and distant metastasis （ P 〈 0.05 ）. Conclusion MMP-7 plays an important role in the genesis ,development ,invasion and metastasis of colorectal carcinoma.