人体恶性肿瘤裸鼠移植药敏试验与PCNA表达的相关性研究

目的 探讨人体恶性肿瘤裸鼠移植瘤药敏试验与ＰＣＮＡ表达的内在联系，方法 新鲜实体肿瘤裸鼠肾包膜下移植３０例，每例２４只ＢＡＬＢ／ｃ裸鼠，随机分为８组（有１０例只分为５或６组），每组２只腹腔注射１种抗癌药，１只注射生理盐水对照；测定药敏结果后移植瘤行组织学和ＰＣＮＡ检测。结果 移植瘤１２２８个，其中对照组４００个，呈正生长６０．３％，无生长２０．８％，负生长１８．９％；检测抗癌药１３种种，敏感５１次

Research of the correlation between drug sensitive testin nude mice malignant tumor transplanted from human and PCNA ex-pression

Objective To study the relationship between drug sensitive test in nude mouse malignant tumor transplanted from human and PCNA expression.Methods 30 cases fresh tumor from human were transplanted under the capsule of kidney, of to 24 BALB/c nude mice, and mice were divided into 8 groups at random. Two mice in each group were treated by abdominal injection of either anticarcinogen or normal saline. Histology and PCNA were examined after drug sensitive test.Results The number of transplantation tumor was 1 288, 400 in control group, with 60.3% of positive growth, 20.8% of no growth, 18.9% of negative growth. 13 kinds of anticarcinogens were detected, with 51 times sensitive, 52 times fairly sensitive, and 90 times insensitive. 288 transplantation tumor were detected under microscope with anoncocyte visible rate of 75.3%. The positive rate of PCNA expression was 69.2% and 70.8% respectively( P >0.05) in primary tumor samples and control group. Conclusion Malignant transplantation tumor in nude mice human from remains its primary biological characteristics. The immunolabelling of PCNA was significantly different between drug sensitivity group and control sensitivity of tumor to drug is negatively correlated with PCNA, the expression of which is related to porognosis.