矩形髓内钉固定对兔胫骨骨折骨痂含量和成骨细胞内骨形态生成蛋白含量影响

目的：探讨钢板和矩形髓内钉固定对兔胫腓骨骨折骨痂含量和成骨细胞内骨形态生成蛋白（BMP）含量的影响。方法：第1部分实验用新西兰兔24只，对同一动物的两侧下肢分别用矩形髓内钉和4孔不锈钢板固定，并分3批于固定后14，21，28 d处死。用免疫组化方法检测骨痂中成骨细胞中BMP的分布，用计算机图像分析系统将结果进行定量分析。第2部分实验用新西兰兔8只，同法制作动物实验模型,于术后56 d摄双侧胫骨X线片，将X线片经计算机图像分析处理系统对骨折处骨痂图像进行定量分析。结果：矩形髓内钉组术后56 d骨痂含量和术后14，21，28 d骨痂中成骨细胞内BMP含量均高于钢板组（P<0.05）。结论:矩形髓内钉内固定胫骨有利于骨折愈合。

Effects of rectangle-shaped intramedullary nailing on the bone callus and the bone morphogenetic protein content in osteoblast

Objective: To understand the effects of rectangle-shaped intramedullary nailing on the bone callus and the bone morphogenetic protein content in osteoblast. Methods: Twenty-four New Zealand rabbits tibial fractures were internally fixed by RIM and plates, animals were killed in groups successively at 14, 21, and 28 d and samples from bone callus of the fracture site were reacted with BMP-Ab by immunochemistry method. And the results were quantified by IAS. X-ray films were taken of both tibia 56 d after operation, and the X-ray films were also quantified by IAS. Results: BMP content in single cell from flexible fixation group is significantly higher than that of plating group, and bone callus content from the RIM fixation group is also richer than that of the plating group. Conclusion: After RIM internal fixation, there is micromovement between the bone fragments, and this may stimulates the secretion of BMP. And the increment of BMP secretion may lead to richer bone callus formation.